Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- AF4330 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse SLAM/CD150 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse SLAM/CD150 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SLAM is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9QUM4
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of SLAM/CD150 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were treated for 2 days with 50 ng/mL PMA and 500 ng/mL Ca2+ ionomycin then stained with Sheep Anti-Mouse SLAM/CD150 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4330, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).