Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF375 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TRAIL/TNFSF10 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TRAIL/TNFSF10 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TRAIL is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P50591
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Hypoxia-inducible factors have distinct and stage-specific roles during reprogramming of human cells to pluripotency.
Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma.
KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis.
Cloning and apoptosis-inducing activities of canine and feline TRAIL.
Osteoprotegerin decreases human osteoclast apoptosis by inhibiting the TRAIL pathway.
Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage.
TNF-related apoptosis-inducing ligand mediates human neuronal apoptosis: links to HIV-1-associated dementia.
Apoptosis-mediating receptor-ligand systems in human retinal pigment epithelial cells.
Mathieu J, Zhou W, Xing Y, Sperber H, Ferreccio A, Agoston Z, Kuppusamy KT, Moon RT, Ruohola-Baker H
Cell stem cell 2014 May 1;14(5):592-605
Cell stem cell 2014 May 1;14(5):592-605
Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma.
Kim SM, Woo JS, Jeong CH, Ryu CH, Jang JD, Jeun SS
Stem cells translational medicine 2014 Feb;3(2):172-82
Stem cells translational medicine 2014 Feb;3(2):172-82
KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis.
Navenot JM, Fujii N, Peiper SC
Molecular pharmacology 2009 May;75(5):1074-83
Molecular pharmacology 2009 May;75(5):1074-83
Cloning and apoptosis-inducing activities of canine and feline TRAIL.
Rong S, Cai JH, Andrews J
Molecular cancer therapeutics 2008 Jul;7(7):2181-91
Molecular cancer therapeutics 2008 Jul;7(7):2181-91
Osteoprotegerin decreases human osteoclast apoptosis by inhibiting the TRAIL pathway.
Chamoux E, Houde N, L'Eriger K, Roux S
Journal of cellular physiology 2008 Aug;216(2):536-42
Journal of cellular physiology 2008 Aug;216(2):536-42
Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage.
Dunn C, Brunetto M, Reynolds G, Christophides T, Kennedy PT, Lampertico P, Das A, Lopes AR, Borrow P, Williams K, Humphreys E, Afford S, Adams DH, Bertoletti A, Maini MK
The Journal of experimental medicine 2007 Mar 19;204(3):667-80
The Journal of experimental medicine 2007 Mar 19;204(3):667-80
TNF-related apoptosis-inducing ligand mediates human neuronal apoptosis: links to HIV-1-associated dementia.
Ryan LA, Peng H, Erichsen DA, Huang Y, Persidsky Y, Zhou Y, Gendelman HE, Zheng J
Journal of neuroimmunology 2004 Mar;148(1-2):127-39
Journal of neuroimmunology 2004 Mar;148(1-2):127-39
Apoptosis-mediating receptor-ligand systems in human retinal pigment epithelial cells.
Hueber A, Aduckathil S, Kociok N, Welsandt G, Dinslage S, Kirchhof B, Esser PJ
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2002 Jul;240(7):551-6
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2002 Jul;240(7):551-6
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TRAIL/TNFSF10 by Western Blot. Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TRAIL/TNFSF10 at approximately 27 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TRAIL/TNFSF10 in Human Brain. TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TRAIL/TNFSF10 in Human Prostate Cancer Tissue. TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TRAIL/TNFSF10 and Neutralization by Human TRAIL/TNFSF10 Antibody. In the presence of a cross-linking antibody, Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050), and the metabolic inhibitor Actinomycin D, Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375). The ND50 is typically 10-60 ng/mL.