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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- RQ5552 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- RP2 Antibody / XRP2
- Antibody type
- Monoclonal
- Description
- Antigen affinity purified
- Reactivity
- Human
- Host
- Mouse
- Antibody clone number
- 3D7
- Vial size
- 100 ug
- Storage
- After reconstitution, the RP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HepG2, 2) Jurkat, 3) Caco-2, 4) HeLa and 5) A549 cell lysate with RP2 antibody. Predicted molecular weight ~40 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with RP2 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human 293T cells with RP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RP2 antibody.
