Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB5218 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD9 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains mouse CD9-transfected cells but not irrelevant transfecants.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P40240
- Isotype
- IgG
- Antibody clone number
- 479608
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD9 in Mouse Kidney. CD9 was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse CD9 Monoclonal Antibody (Catalog # MAB5218) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD9 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CD9 Monoclonal Antibody (Catalog # MAB5218, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG APC-conjugated antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.