14-0489-80

antibody from Invitrogen Antibodies

Targeting: CD48 BCM1, BLAST, hCD48, mCD48, SLAMF2

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14-0489-80

Provider

Invitrogen Antibodies

Product name

CD48 Monoclonal Antibody (eBio156-4H9 (156-4H9)), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The eBio156-4H9 monoclonal antibody reacts with the human CD48 antigen, also known as B cell activation marker 1, SLAM family member 2, Blast-1 (human), and OX-45 (rat). CD48, a member of the SLAM family and Ig superfamily, is a 45 kDa GPI-linked glycoprotein expressed on the majority of hematopoietic cells. CD2 is the low affinity ligand for CD48.Recent publications have reported differential expression in mice of members of the SLAM family including CD48, CD150, and CD244 among functionally distinct bone marrow hematopoietic progenitors providing a useful tool for prediction of the primitiveness of hematopoietic progenitors. Expression of CD48 in hematopoietic cells is found on many leukocytes with little to none on granulocytes and absent on platelets and erythrocytes. Expression increases on B cells with activation.

Antibody clone number

eBio156-4H9 (156-4H9)

Concentration

0.5 mg/mL

References

Submitted references

Cytomegalovirus-Induced Expression of CD244 after Liver Transplantation Is Associated with CD8+ T Cell Hyporesponsiveness to Alloantigen.

de Mare-Bredemeijer EL, Shi XL, Mancham S, van Gent R, van der Heide-Mulder M, de Boer R, Heemskerk MH, de Jonge J, van der Laan LJ, Metselaar HJ, Kwekkeboom J
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1838-48

---

WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

Xia P, Wang S, Huang G, Zhu P, Li M, Ye B, Du Y, Fan Z
The Journal of experimental medicine 2014 Sep 22;211(10):2119-34

---

IL-7Rαlow memory CD8+ T cells are significantly elevated in patients with systemic lupus erythematosus.

Kim JS, Cho BA, Sim JH, Shah K, Woo CM, Lee EB, Lee DS, Kang JS, Lee WJ, Park CG, Craft J, Kang I, Kim HR
Rheumatology (Oxford, England) 2012 Sep;51(9):1587-94

---

Expression of the Blast-1 activation/adhesion molecule and its identification as CD48.

Yokoyama S, Staunton D, Fisher R, Amiot M, Fortin JJ, Thorley-Lawson DA
Journal of immunology (Baltimore, Md. : 1950) 1991 Apr 1;146(7):2192-200

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood mononuclear cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human CD48 Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Cells in the lymphocyte gate were used for analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. WASH deficiency impairs repopulation capacity of HSCs. (A) Flow cytometry of HSCs in Ctrl and WASH KO mice. Sca-1 + c-Kit + cells in the Lin - and IL-7Ralpha - population were gated out (top left) for Flk2 analysis (bottom left). Percentages of the indicated cells in parent populations were calculated and shown on the right. IgG was gated as a negative control. n = 15 for each group. (B) Survival curves of CD45.1 mice transplanted with BM cells from Ctrl ( n = 20) and KO mice ( n = 21). Lethally irradiated CD45.1 mice were transplanted with BM cells from Ctrl and KO mice (left) and survival rates were calculated (right). Lethally irradiated CD45.1 mice without transfer served as a negative control ( n = 13). (C) Equal amounts of CD45.1 (CFSE labeled) and CD45.2 (Violet labeled) lin - BM cells were transplanted into lethally irradiated CD45.1 mice, followed by flow cytometry 18 h after transplantation. Homing efficiency was calculated as: absolute number of Violet + lin - cells 18 h after transplantation/absolute number of Violet + Lin - cells transplanted. n = 10 per group. (D and E) 5 x 10 6 BM cells from MxCre - ;WASH flox/flox and MxCre + ;WASH flox/flox mice were transplanted into lethally irradiated CD45.1 mice. 8 wk later, poly(I:C) was administrated as described in Materials and methods, followed by flow cytometry of the indicated cells 8 wk after poly(I:C) injection (D). Otherwise, 5 x 10 6 BM cells from CD45.1 mice were transplanted into lethally irradiated