Antibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
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- Product number
- 16-0489-85 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD48 Monoclonal Antibody (eBio156-4H9 (156-4H9)), Functional Grade, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBio156-4H9 monoclonal antibody reacts with the human CD48 antigen, also known as B cell activation marker 1, SLAM family member 2, Blast-1 (human), and OX-45 (rat). CD48, a member of the SLAM family and Ig superfamily, is a 45 kDa GPI-linked glycoprotein expressed on the majority of hematopoietic cells. CD2 is the low affinity ligand for CD48.Recent publications have reported differential expression in mice of members of the SLAM family including CD48, CD150, and CD244 among functionally distinct bone marrow hematopoietic progenitors providing a useful tool for prediction of the primitiveness of hematopoietic progenitors. Expression of CD48 in hematopoietic cells is found on many leukocytes with little to none on granulocytes and absent on platelets and erythrocytes. Expression increases on B cells with activation.
- Antibody clone number
- eBio156-4H9 (156-4H9)
- Concentration
- 1 mg/mL
Submitted references WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.
IL-1β production through the NLRP3 inflammasome by hepatic macrophages links hepatitis C virus infection with liver inflammation and disease.
Simultaneous TCR and CD244 signals induce dynamic downmodulation of CD244 on human antiviral T cells.
Xia P, Wang S, Huang G, Zhu P, Li M, Ye B, Du Y, Fan Z
The Journal of experimental medicine 2014 Sep 22;211(10):2119-34
The Journal of experimental medicine 2014 Sep 22;211(10):2119-34
IL-1β production through the NLRP3 inflammasome by hepatic macrophages links hepatitis C virus infection with liver inflammation and disease.
Negash AA, Ramos HJ, Crochet N, Lau DT, Doehle B, Papic N, Delker DA, Jo J, Bertoletti A, Hagedorn CH, Gale M Jr
PLoS pathogens 2013;9(4):e1003330
PLoS pathogens 2013;9(4):e1003330
Simultaneous TCR and CD244 signals induce dynamic downmodulation of CD244 on human antiviral T cells.
Pacheco Y, McLean AP, Rohrbach J, Porichis F, Kaufmann DE, Kavanagh DG
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 1;191(5):2072-81
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 1;191(5):2072-81
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 3. WASH deficiency impairs repopulation capacity of HSCs. (A) Flow cytometry of HSCs in Ctrl and WASH KO mice. Sca-1 + c-Kit + cells in the Lin - and IL-7Ralpha - population were gated out (top left) for Flk2 analysis (bottom left). Percentages of the indicated cells in parent populations were calculated and shown on the right. IgG was gated as a negative control. n = 15 for each group. (B) Survival curves of CD45.1 mice transplanted with BM cells from Ctrl ( n = 20) and KO mice ( n = 21). Lethally irradiated CD45.1 mice were transplanted with BM cells from Ctrl and KO mice (left) and survival rates were calculated (right). Lethally irradiated CD45.1 mice without transfer served as a negative control ( n = 13). (C) Equal amounts of CD45.1 (CFSE labeled) and CD45.2 (Violet labeled) lin - BM cells were transplanted into lethally irradiated CD45.1 mice, followed by flow cytometry 18 h after transplantation. Homing efficiency was calculated as: absolute number of Violet + lin - cells 18 h after transplantation/absolute number of Violet + Lin - cells transplanted. n = 10 per group. (D and E) 5 x 10 6 BM cells from MxCre - ;WASH flox/flox and MxCre + ;WASH flox/flox mice were transplanted into lethally irradiated CD45.1 mice. 8 wk later, poly(I:C) was administrated as described in Materials and methods, followed by flow cytometry of the indicated cells 8 wk after poly(I:C) injection (D). Otherwise, 5 x 10 6 BM cells from CD45.1 mice were transplanted into lethally irradiated