MA1-19030

antibody from Invitrogen Antibodies

Targeting: ICAM3 CD50, CDW50, ICAM-R

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-19030

Provider

Invitrogen Antibodies

Product name

ICAM-3 Monoclonal Antibody (MEM-04)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody reacts with an extracellular epitope of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes. The MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1). Functional Application: Blocking.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

MEM-04

Vial size

100 µg

Concentration

1 mg/mL

Storage

4°C, do not freeze

References

Submitted references

Increased Expressions of ICAM-2 and ICAM-3 in Pterygium.

Demiryürek S, Saracaloglu A, Kimyon S, Mete A, Eronat O, Temiz E, Nacarkahya G, Tunca ZS, Düzen B, Saygili O, Güngör K, Karakök M, Demiryürek AT
Current eye research 2019 Jun;44(6):645-650

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry staining of human tonsil (paraffin sections) using anti-CD50 (MEM-04) Monoclonal antibody (Product # MA1-19030).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of ICAM-3 using ICAM-3 Monoclonal Antibody (MEM-04) (Product # MA1-19030). Separation of leukocytes stained using anti-human CD50 (MEM-04) purified antibody (concentration in sample 3 μg/mL, GAM APC, red-filled) from leukocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of ICAM-3 using ICAM-3 Monoclonal Antibody (MEM-04) (Product # MA1-19030). Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human ICAM-3 (MEM-04) purified antibody (concentration in sample 3 μg/mL, GAM APC).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. Immunohistochemical images of ICAM-2 and ICAM-3 staining. Immunostaining with ICAM-2 (A and B) and ICAM-3 (C and D) for human normal conjunctiva (A and C) and pterygia (B and D). Scale bar = 200 mm.