AM33037PU-N

antibody from OriGene

Targeting: ICAM3 CD50, CDW50, ICAM-R

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM33037PU-N

Provider

OriGene

Product name

ICAM3 mouse monoclonal antibody, clone JA4, Purified

Antibody type

Monoclonal

Description

ICAM3 mouse monoclonal antibody, clone JA4, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

ICAM3

Isotype

IgG

Antibody clone number

JA4

Vial size

100 µg

Concentration

1.0 mg/ml

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Jurkat T cells that expressed high (ICAM-3high= red) or low (ICAM-3low= blue) levels of ICAM-3 were stained with JA4. 5 x 10⁵ cells were stained with 100ul of mAb. Following 30 min incubation at 4°C, unbound Ab was removed by washing and bound mAb detected by staining with goat antimouse- FITC for 30 min at 4°C. Flow cytometric histogram of 5000 events per sample is shown.

Validation comment

FC