MAB6936

antibody from Abnova Corporation

Targeting: ICAM3 CD50, CDW50, ICAM-R

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6936

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB6936, RRID:AB_10676912

Product name

ICAM3 monoclonal antibody, clone 186-2G9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against native ICAM3.

Isotype

IgG

Antibody clone number

186-2G9

Storage

Store at 4°C.

References

Submitted references

Critical amino acids in the lymphocyte function-associated antigen-1 I domain mediate intercellular adhesion molecule 3 binding and immune function.

van Kooyk Y, Binnerts ME, Edwards CP, Champe M, Berman PW, Figdor CG, Bodary SC
The Journal of experimental medicine 1996 Mar 1;183(3):1247-52

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Analysis of the binding site on intercellular adhesion molecule 3 for the leukocyte integrin lymphocyte function-associated antigen 1.

Holness CL, Bates PA, Little AJ, Buckley CD, McDowall A, Bossy D, Hogg N, Simmons DL
The Journal of biological chemistry 1995 Jan 13;270(2):877-84

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

5 X 10⁵ cultured human Jurkat cells per tube were washed and preincubated ~5 minutes on ice with 20 ml of 250 mg/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ml of ICAM3 monoclonal antibody, clone 186-2G9 (Cat # MAB6936) at 10 mg/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.84 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration.

Validation comment

Flow Cytometry