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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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- Product number
- Ab006583 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- CD57 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human CD57 Antibody, Monoclonal (HNK-1), could be used for Flow and so on.Application:Flow: 0.125_g/100w cells Protein FunctionInvolved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- HNK-1
- Vial size
- 100_g,10_g,1mg,50_g
- Concentration
- Lot by Lot
- Storage
- Shipped at 4¡C. Store at 4¡C long term (12 months). Upon delivery aliquot.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD57 Mouse mAb (Ab006583) - Flow Cytometry Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD57 with CD57 Mouse mAb (Ab006583) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD57 Mouse mAb (Ab006583) - Flow Cytometry Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD57 (red) with CD57 Mouse mAb (Ab006583) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
