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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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- Product number
- FAB2386A - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD69 APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD69 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Red dye
- Antigen sequence
P37217- Isotype
- IgG
- Antibody clone number
- 310106
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD69 in CTLL-2 Mouse Cell Line by Flow Cytometry. CTLL-2 mouse cytotoxic T cell line was stained with Rat Anti-Mouse CD69 APC-conjugated Monoclonal Antibody (Catalog # FAB2386A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD69 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were (A) cultured with plate-bound anti-CD3 and anti-CD28 antibodies overnight, or (B) unstimulated, then stained with Rat Anti-Mouse CD69 APC-conjugated Monoclonal Antibody (Catalog # FAB2386A) and Rat anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on isotype control antibody (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
