- MHCD6905 - Invitrogen Antibodies CD69 antibody

Submitted references Novel lectin-based chimeric antigen receptors target Gb3-positive tumour cells.

Meléndez AV, Velasco Cárdenas RM, Lagies S, Strietz J, Siukstaite L, Thomas OS, Tomisch J, Weber W, Kammerer B, Römer W, Minguet S
Cellular and molecular life sciences : CMLS 2022 Sep 12;79(10):513

Conformational surveillance of Orai1 by a rhomboid intramembrane protease prevents inappropriate CRAC channel activation.

Grieve AG, Yeh YC, Chang YF, Huang HY, Zarcone L, Breuning J, Johnson N, Stříšovský K, Brown MH, Parekh AB, Freeman M
Molecular cell 2021 Dec 2;81(23):4784-4798.e7

Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor.

Yousefi OS, Günther M, Hörner M, Chalupsky J, Wess M, Brandl SM, Smith RW, Fleck C, Kunkel T, Zurbriggen MD, Höfer T, Weber W, Schamel WW
eLife 2019 Apr 5;8

Reduction of leucocyte cell surface disulfide bonds during immune activation is dynamic as revealed by a quantitative proteomics workflow (SH-IQ).

Stegmann M, Barclay AN, Metcalfe C
Open biology 2018 Sep 19;8(9)

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5. Thiol levels increase on the leucocyte cell surface during immune activation. PBMCs of two donors were mixed at a 1 : 1 ratio and co-cultured for 96 h. ( a ) Cells were removed at the time points indicated for flow cytometry analysis and thiols visualized by Alexa-488-maleimide staining. ( b ) Quantitative analysis of flow cytometry data shown in ( a ). Values of four biological replicates relative to time point 0 h +- standard deviation. Example raw flow cytometry data gated on T cells (expressing alpha/beta TCR chain) showing the increase of thiol levels (Alexa-488-maleimide) correlates to T cell activation (increase in CD69 expression) ( c ) at time 0 and ( d ) after 96 h. ( e ) Quantitative analysis of flow cytometry data at time points taken throughout the MLR. Normalized mean values relative to time point 0 h for three replicates +- s.e. of the mean.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 RHBDL2 regulates human T cell activation (A) TaqMan assays for RHBDL2 mRNA levels in T cells transduced with virus encoding control or RHBDL2 shRNAs. Error bars represent RQ standard error. (B) T cell activation measured by quantification of surface CD69 expression by fluorescence-activated cell sorting (FACS). CD69 expression is compared between control and RHBDL2 shRNA transduced primary CD4 T cells after stimulation with CD3. Each trace represents three biological replicates. In the dashed box, the calculated EC 50 of anti-CD3 for each shRNA. Error indicates SEM. (C) SOCE is monitored by cytosolic Fura-2 fluorescence and compared between control and RHBDL2 shRNA transduced T cells. T cells were stimulated with 2 muM thapsigargin in Ca 2+ -free buffer, followed by readmission of 2 mM external Ca 2+ . (D and E) Aggregate data from T cells treated as in (C) are plotted, analyzing the peak Ca 2+ level in each condition (D) and rate of Ca 2+ entry (E). Each bar in (D) and (E) represents between 34 and 45 cells. For two-tailed t tests, *** p < 0.001, compared with control shRNA transduced T cells. Error bars represent SEM.

MHCD6905

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