MA1-207

Boardman DA, Philippeos C, Fruhwirth GO, Ibrahim MA, Hannen RF, Cooper D, Marelli-Berg FM, Watt FM, Lechler RI, Maher J, Smyth LA, Lombardi G
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2017 Apr;17(4):931-943

C1q acts in the tumour microenvironment as a cancer-promoting factor independently of complement activation.

Bulla R, Tripodo C, Rami D, Ling GS, Agostinis C, Guarnotta C, Zorzet S, Durigutto P, Botto M, Tedesco F
Nature communications 2016 Feb 1;7:10346

Aberrant germinal center formation, follicular T-helper cells, and germinal center B-cells were involved in chronic graft-versus-host disease.

Shao L, Lie AK, Zhang Y, Wong CH, Kwong YL
Annals of hematology 2015 Sep;94(9):1493-504

Effector CD4 T-cell transition to memory requires late cognate interactions that induce autocrine IL-2.

McKinstry KK, Strutt TM, Bautista B, Zhang W, Kuang Y, Cooper AM, Swain SL
Nature communications 2014 Nov 5;5:5377

Lysophosphatidic acid receptor 5 inhibits B cell antigen receptor signaling and antibody response.

Hu J, Oda SK, Shotts K, Donovan EE, Strauch P, Pujanauski LM, Victorino F, Al-Shami A, Fujiwara Y, Tigyi G, Oravecz T, Pelanda R, Torres RM
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 1;193(1):85-95

Arhgef1 is required by T cells for the development of airway hyperreactivity and inflammation.

Brown JP, Taube C, Miyahara N, Koya T, Pelanda R, Gelfand EW, Torres RM
American journal of respiratory and critical care medicine 2007 Jul 1;176(1):10-9

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CD69 was performed by loading 20 µg of indicated whole cell lysates in reducing sample buffer and 8 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX). Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. CD69 was detected at ~30 kDa using a CD69 hamster monoclonal antibody (Product # MA1-207) at a concentration of 1 µg/mL in blocking buffer overnight at 4°C on a rocking platform, followed by a HRP conjugated secondary antibody (Product # PA1-28823) at a dilution of 1:5000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura Extended Duration Substrate (Product # 34076) and the myECL Imager (Product # 62236).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CD69 (green) in PHA-M (2% v/v, Product # 10576-015) treated Raji cells. Cells were blocked with blocking buffer (Product # 37525) for 30 min at room temparture and then stained with CD69 hamster monoclonal antibody (Product # MA1-207) at a dilution of 1:100 for 1 hour on ice. After washing with ice-cold PBS for 3 times, the cells were then stained with DyLight 488 Goat anti-hamster (Armenian) IgG antibody for 1 hour on ice. The cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature. Nuclei (blue) were counterstained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD69 on Raji cells with/without the treatment of PHA-M (Product # 10576-015) as indicated for overnight. Cells were blocked with blocking buffer (Product # 37525) for 30 min at room temparture and then stained with CD69 hamster monoclonal antibody (Product # MA1-207) at a dilution of 1:100 for 1 hour on ice. After washing with ice-cold PBS for 3 times, the cells were stained with DyLight 488 Goat anti-hamster (Armenian) IgG antibody for 1 hour on ice. A representative 10,000 cells were acquired for each sample.