Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-4110 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-CD69 Antibody
- Antibody type
- Monoclonal
- Description
- CD69 is a type II transmembrane protein of the C-type lectin family and its gene is located within the natural killer gene complex. In healthy subjects, CD69 is not detected in peripheral blood lymphocytes, but is expressed on small subsets of T and B cells in peripheral lymphoid tissues. CD69 gene maps at human chromosome 12, and behaves as an early activation gene that contains responsive elements for the transcription factors NF-kappaB, ERG-1 and AP-1. This protein acts as a leukocyte activation marker, and is involved in the activation of different leukocyte subsets as well as in the pathogenesis of chronic inflammation.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Full length recombinant CD69 protei
n was used as the immunogen for thi
s antibody.- Isotype
- IgG
- Antibody clone number
- ABM39A4
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of CD69. Anti- CD69 antibody (Clone: ABM39A4) was used at 4 µg/ml on (1)Daudi and (2) h Lung lysates.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of CD69. Anti- CD69 antibody (Clone: ABM39A4) was used at 2 µg/ml on U937 lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD69 in human kidney tissue using CD69 antibody (Clone: ABM39A4) at 5 µg/ml.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD69 in human Prostate tissue using CD69 antibody (Clone: ABM39A4) at 5 µg/ml.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Cell surface FLOW analysis of hCD69 (10-4110) in PBMC treated 3 days with PMA + Ionomycin (Lymphocytes gated) using 0.5 ug antibody per 10^6 cells. Green represents PBMC W/O treatment, red represents PBMC with treatment. Goat anti-mouse PE conjugated secondary antibody was used (ABEOMICS).
- Protocol
- Protocol