LS-C818906

antibody from LSBio

Targeting: CD69 CLEC2C

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C818906

Provider

LSBio

Product name

CD69 Antibody (clone 074, Preservative Free) LS-C818906

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

074

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-CD69 rabbit monoclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: HL60 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

CD69 was immunoprecipitated using: Lane A: 0.5 mg HL-60 Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. 0.5 uL anti-CD69 rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CD69 rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CD69 was immunoprecipitated using: Lane A: 0.5 mg HL-60 Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. 0.5 uL anti-CD69 rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CD69 rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa.