Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 50-0729-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD72 Monoclonal Antibody (J4-117), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody J4-117 reacts with human CD72, a 45 kDa type II transmembrane glycoprotein that belongs to the C-type lectin superfamily. This glycoprotein is expressed on B cells at all stages of maturation except for plasma cells. CD72 is also expressed on dendritic cells, mast cells, and some macrophages. This receptor contains an immune-receptor tyrosine-base inhibition motif (ITIM) that associates with a tyrosine phosphatase, SHP-1. CD72 negatively regulates the major signal transduction pathways activated by the B cell receptor (BCR). Studies have demonstrated that the ligand for CD72 is CD100 (also known as Semaphorin 4D). CD72 can also react with CD5 to provide signaling between T and B cells. Finally, this receptor also prevents naive B cells from producing Ig and differentiating into B cells, and plays a role in controlling autoimmunity by enhancing apoptosis after Ag-specific BCR stimulation. Applications Reported: This J4-117 antibody has been reported for use in flow cytometric analysis. Applications Tested: This J4-117 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- J4-117
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Human CD72 splicing isoform responsible for resistance to systemic lupus erythematosus regulates serum immunoglobulin level and is localized in endoplasmic reticulum.
CD72 negatively regulates KIT-mediated responses in human mast cells.
CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72.
CD72 down-modulates BCR-induced signal transduction and diminishes survival in primary mature B lymphocytes.
Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment.
CD72-mediated suppression of human naive B cell differentiation by down-regulating X-box binding protein 1.
A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5.
Hitomi Y, Adachi T, Tsuchiya N, Honda Z, Tokunaga K, Tsubata T
BMC immunology 2012 Dec 26;13:72
BMC immunology 2012 Dec 26;13:72
CD72 negatively regulates KIT-mediated responses in human mast cells.
Kataoka TR, Kumanogoh A, Bandara G, Metcalfe DD, Gilfillan AM
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2468-75
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2468-75
CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72.
Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O
PloS one 2007 Sep 5;2(9):e818
PloS one 2007 Sep 5;2(9):e818
CD72 down-modulates BCR-induced signal transduction and diminishes survival in primary mature B lymphocytes.
Li DH, Tung JW, Tarner IH, Snow AL, Yukinari T, Ngernmaneepothong R, Martinez OM, Parnes JR
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5321-8
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5321-8
Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment.
Kumanogoh A, Shikina T, Watanabe C, Takegahara N, Suzuki K, Yamamoto M, Takamatsu H, Prasad DV, Mizui M, Toyofuku T, Tamura M, Watanabe D, Parnes JR, Kikutani H
International immunology 2005 Oct;17(10):1277-82
International immunology 2005 Oct;17(10):1277-82
CD72-mediated suppression of human naive B cell differentiation by down-regulating X-box binding protein 1.
Yamazaki T, Nagumo H, Hayashi T, Sugane K, Agematsu K
European journal of immunology 2005 Aug;35(8):2325-34
European journal of immunology 2005 Aug;35(8):2325-34
A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5.
Bikah G, Lynd FM, Aruffo AA, Ledbetter JA, Bondada S
International immunology 1998 Aug;10(8):1185-96
International immunology 1998 Aug;10(8):1185-96
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (Product # 11-0199-42) and Mouse IgG2b K Isotype Control eFluor® 660 (Product # 50-4732-82) (left) or Anti-Human CD72 eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.