50-0729-42

antibody from Invitrogen Antibodies
Targeting: CD72 CD72b, LYB2
Provider product page for 50-0729-42

Antibody data

Product number
50-0729-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD72 Monoclonal Antibody (J4-117), eFluor™ 660, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody J4-117 reacts with human CD72, a 45 kDa type II transmembrane glycoprotein that belongs to the C-type lectin superfamily. This glycoprotein is expressed on B cells at all stages of maturation except for plasma cells. CD72 is also expressed on dendritic cells, mast cells, and some macrophages. This receptor contains an immune-receptor tyrosine-base inhibition motif (ITIM) that associates with a tyrosine phosphatase, SHP-1. CD72 negatively regulates the major signal transduction pathways activated by the B cell receptor (BCR). Studies have demonstrated that the ligand for CD72 is CD100 (also known as Semaphorin 4D). CD72 can also react with CD5 to provide signaling between T and B cells. Finally, this receptor also prevents naive B cells from producing Ig and differentiating into B cells, and plays a role in controlling autoimmunity by enhancing apoptosis after Ag-specific BCR stimulation. Applications Reported: This J4-117 antibody has been reported for use in flow cytometric analysis. Applications Tested: This J4-117 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
J4-117
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
CD72 protein structure - 50-0729-42 shown in red.