H00003732-M01

antibody from Abnova Corporation

Targeting: CD82 IA4, KAI1, R2, ST6, TSPAN27

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003732-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003732-M01, RRID:AB_622284

Product name

CD82 monoclonal antibody (M01), clone 4F2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant CD82.

Antigen sequence

MGSACIKVTKYFLFLFNLIFFILGAVILGFGVWIL
ADKSSFISVLQTSSSSLRMGAYVFIGVGAVTMLMG
FLGCIGAVNEVRCLLGLYFAFLLLILIAQVTAGAL
FYFNMGKLKQEMGGIVTELIRDYNSSREDSLQDAW
DYVQAQVKCCGWVSFYNWTDNAELMNRPEVTYPCS
CEVKGEEDNSLSVRKGFCEAPGNRTQSGNHPEDWP
VYQEGCMEKVQAWLQENLGIILGVGVGVAIVELLG
MVLSICLCRHVHSEDYSKVPKY


Isotype

IgG

Antibody clone number

4F2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CD82 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between CD19 and CD82. HeLa cells were stained with anti-CD19 rabbit purified polyclonal 1:1200 and anti-CD82 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)