Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PAB4830 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4830, RRID:AB_1572351
- Product name
- CD82 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CD82.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references [Role of tumor metastasis suppressor gene KAI1 in development of colorectal cancer].
EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits the migration of prostate cancer cells.
Requirement of the p130CAS-Crk coupling for metastasis suppressor KAI1/CD82-mediated inhibition of cell migration.
Yang J, Liu FX, Yan XC, He GY, Liu LM
Ai zheng = Aizheng = Chinese journal of cancer 2003 May;22(5):533-6
Ai zheng = Aizheng = Chinese journal of cancer 2003 May;22(5):533-6
EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits the migration of prostate cancer cells.
Zhang XA, Lane WS, Charrin S, Rubinstein E, Liu L
Cancer research 2003 May 15;63(10):2665-74
Cancer research 2003 May 15;63(10):2665-74
Requirement of the p130CAS-Crk coupling for metastasis suppressor KAI1/CD82-mediated inhibition of cell migration.
Zhang XA, He B, Zhou B, Liu L
The Journal of biological chemistry 2003 Jul 18;278(29):27319-28
The Journal of biological chemistry 2003 Jul 18;278(29):27319-28
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The CD82 polyclonal antibody (Cat # PAB4830) is used in Western blot to detect CD82 in mouse liver tissue lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with CD82 polyclonal antibody (Cat # PAB4830) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using CD82 polyclonal antibody (Cat # PAB4830)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry