Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 11-0839-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD83 Monoclonal Antibody (HB15e), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HB15e monoclonal antibody reacts with human CD83, a 45 kDa transmembrane glycoprotein. CD83, a member of the Ig superfamily, is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells as well as some proliferating lymphocytes, and human cell lines express this antigen. While the function of CD83 is unclear, it can serve as a useful marker for mature human blood dendritic cells. Applications Reported: The HB15e antibody has been reported for use in flow cytometric analysis. Applications Tested: This HB15e antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- HB15e
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references STAT3 phosphorylation at Ser727 and Tyr705 differentially regulates the EMT-MET switch and cancer metastasis.
Herpes Simplex Virus Type-2 Paralyzes the Function of Monocyte-Derived Dendritic Cells.
Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.
TNF blockade induces a dysregulated type I interferon response without autoimmunity in paradoxical psoriasis.
Dexamethasone and Monophosphoryl Lipid A Induce a Distinctive Profile on Monocyte-Derived Dendritic Cells through Transcriptional Modulation of Genes Associated With Essential Processes of the Immune Response.
Hepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism.
Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.
Novel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses.
Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen.
Early secreted antigenic target of 6-kDa protein of Mycobacterium tuberculosis primes dendritic cells to stimulate Th17 and inhibit Th1 immune responses.
Dendritic cells derived from hemozoin-loaded monocytes display a partial maturation phenotype that promotes HIV-1 trans-infection of CD4+ T cells and virus replication.
Lin WH, Chang YW, Hong MX, Hsu TC, Lee KC, Lin C, Lee JL
Oncogene 2021 Jan;40(4):791-805
Oncogene 2021 Jan;40(4):791-805
Herpes Simplex Virus Type-2 Paralyzes the Function of Monocyte-Derived Dendritic Cells.
Grosche L, Mühl-Zürbes P, Ciblis B, Krawczyk A, Kuhnt C, Kamm L, Steinkasserer A, Heilingloh CS
Viruses 2020 Jan 16;12(1)
Viruses 2020 Jan 16;12(1)
Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.
Tong D, Zhang L, Ning F, Xu Y, Hu X, Shi Y
Protein & cell 2020 Feb;11(2):108-123
Protein & cell 2020 Feb;11(2):108-123
TNF blockade induces a dysregulated type I interferon response without autoimmunity in paradoxical psoriasis.
Conrad C, Di Domizio J, Mylonas A, Belkhodja C, Demaria O, Navarini AA, Lapointe AK, French LE, Vernez M, Gilliet M
Nature communications 2018 Jan 2;9(1):25
Nature communications 2018 Jan 2;9(1):25
Dexamethasone and Monophosphoryl Lipid A Induce a Distinctive Profile on Monocyte-Derived Dendritic Cells through Transcriptional Modulation of Genes Associated With Essential Processes of the Immune Response.
García-González PA, Schinnerling K, Sepúlveda-Gutiérrez A, Maggi J, Mehdi AM, Nel HJ, Pesce B, Larrondo ML, Aravena O, Molina MC, Catalán D, Thomas R, Verdugo RA, Aguillón JC
Frontiers in immunology 2017;8:1350
Frontiers in immunology 2017;8:1350
Hepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism.
van Montfoort N, van der Aa E, van den Bosch A, Brouwers H, Vanwolleghem T, Janssen HLA, Javanbakht H, Buschow SI, Woltman AM
Journal of virology 2016 Jul 15;90(14):6187-6199
Journal of virology 2016 Jul 15;90(14):6187-6199
Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.
Miles B, Zakhary I, El-Awady A, Scisci E, Carrion J, O'Neill JC, Rawlings A, Stern JK, Susin C, Cutler CW
Infection and immunity 2014 Jan;82(1):101-11
Infection and immunity 2014 Jan;82(1):101-11
Novel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses.
Preston SG, Majtán J, Kouremenou C, Rysnik O, Burger LF, Cabezas Cruz A, Chiong Guzman M, Nunn MA, Paesen GC, Nuttall PA, Austyn JM
PLoS pathogens 2013;9(6):e1003450
PLoS pathogens 2013;9(6):e1003450
Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen.
Miles B, Scisci E, Carrion J, Sabino GJ, Genco CA, Cutler CW
Journal of leukocyte biology 2013 Aug;94(2):281-9
Journal of leukocyte biology 2013 Aug;94(2):281-9
Early secreted antigenic target of 6-kDa protein of Mycobacterium tuberculosis primes dendritic cells to stimulate Th17 and inhibit Th1 immune responses.
Wang X, Barnes PF, Huang F, Alvarez IB, Neuenschwander PF, Sherman DR, Samten B
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 15;189(6):3092-103
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 15;189(6):3092-103
Dendritic cells derived from hemozoin-loaded monocytes display a partial maturation phenotype that promotes HIV-1 trans-infection of CD4+ T cells and virus replication.
Diou J, Tardif MR, Barat C, Tremblay MJ
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):2899-907
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):2899-907
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of 1 day PHA-stimulated human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD83 FITC (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Identification of a gene expression signature linked to early dissemination. A Microarray time-series data analysis was performed on cells after treatment with BM-MSC-CM at 9 time points, from 0 to 96 h. Representative clusters of the indicated genes are shown as heatmaps, with red indicating increased expression and green indicating decreased expression, as indicated by the color intensity scale shown below each heatmap. B Functional fractionation of A549 cells via invasion assays. The percentage of cells expressing surface markers was determined by flow cytometry (CD133 + /CD83 + for early-response cells; CD151 + /CD38 + for late-response cells). C - E Functional fractionation of cancer cells via serial invasion assays. Cells were serially selected through 5 (HM5), 10 (HM10), or 20 (HM20) Matrigel invasion assays. The percentage of cells expressing surface markers was determined by QPCR (CD133 + /CD83 + in D ; CD151 + /CD38 + in E ). F QPCR showing the mRNA expression levels of STAT3 signaling-related genes. HM20 cells are compared to LM cells.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 PA T encounter with DCs results in limited activation involving upregulation of Il2 and Bcl2 . (A) PA T cells were co-cultured with DC1940 for 24 h, then CD4 + populations were gated into CD69 + and CD69 - cells by FACS. The expression of CD103, CXCR5, CD62L, CCR9, CCR7, CD127 and CD83 between CD69 + and CD69 - cells were compared. Three replicates in each group ( n = 3), and results are representative of three independent experiments ( N = 3). (B) CD69 + and CD69 - cells cultured in complete RPMI for 24 h without additional treatment, then the cytokines released to the supernatant were measured by ELISA. Pooled data from three independent experiments are shown. (C) Gene expression of Il7r , Il2 and Bcl2 was determined by QPCR in PA T cells or in CD69 positive or negative populations purified by FACS sorting. Three replicates in each group ( n = 3), and results are representative of three independent experiments ( N = 3). GAPDH was used for normalization
- Conjugate
- Green dye