Antibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [3]
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- Product number
- 12-0839-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD83 Monoclonal Antibody (HB15e), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HB15e monoclonal antibody reacts with human CD83, a 45 kDa transmembrane glycoprotein. CD83, a member of the Ig superfamily, is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells as well as some proliferating lymphocytes, and human cell lines express this antigen. While the function of CD83 is unclear, it can serve as a useful marker for mature human blood dendritic cells. Applications Reported: The HB15e antibody has been reported for use in flow cytometric analysis. Applications Tested: This HB15e antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- HB15e
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references STAT3 phosphorylation at Ser727 and Tyr705 differentially regulates the EMT-MET switch and cancer metastasis.
Recombinant human IL-37 inhibited endometriosis development in a mouse model through increasing Th1/Th2 ratio by inducing the maturation of dendritic cells.
Breast cancer stem cell RNA-pulsed dendritic cells enhance tumor cell killing by effector T cells.
Herpes Simplex Virus Type-2 Paralyzes the Function of Monocyte-Derived Dendritic Cells.
Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.
Endogenous Expression of the Human CD83 Attenuates EAE Symptoms in Humanized Transgenic Mice and Increases the Activity of Regulatory T Cells.
Role of Nuclear Factor (Erythroid-Derived 2)-Like 2 Signaling for Effects of Fumaric Acid Esters on Dendritic Cells.
ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.
In vitro and in vivo studies of the immunomodulatory effect of Echinacea purpurea on dendritic cells.
HIV-1 and HIV-2 differentially mature plasmacytoid dendritic cells into IFN-producing cells or APCs.
Modulation of HIV-1-induced activation of plasmacytoid dendritic cells by 6-desfluoroquinolones.
Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen.
Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling.
Penicillium marneffei-stimulated dendritic cells enhance HIV-1 trans-infection and promote viral infection by activating primary CD4+ T cells.
Immunological aspects of REIC/Dkk-3 in monocyte differentiation and tumor regression.
Decreased migration of Langerhans precursor-like cells in response to human keratinocytes expressing human papillomavirus type 16 E6/E7 is related to reduced macrophage inflammatory protein-3alpha production.
Lin WH, Chang YW, Hong MX, Hsu TC, Lee KC, Lin C, Lee JL
Oncogene 2021 Jan;40(4):791-805
Oncogene 2021 Jan;40(4):791-805
Recombinant human IL-37 inhibited endometriosis development in a mouse model through increasing Th1/Th2 ratio by inducing the maturation of dendritic cells.
Li L, Liao Z, Ye M, Jiang J
Reproductive biology and endocrinology : RB&E 2021 Aug 24;19(1):128
Reproductive biology and endocrinology : RB&E 2021 Aug 24;19(1):128
Breast cancer stem cell RNA-pulsed dendritic cells enhance tumor cell killing by effector T cells.
Sumransub N, Jirapongwattana N, Jamjuntra P, Thongchot S, Chieochansin T, Yenchitsomanus PT, Thuwajit P, Warnnissorn M, O-Charoenrat P, Thuwajit C
Oncology letters 2020 Mar;19(3):2422-2430
Oncology letters 2020 Mar;19(3):2422-2430
Herpes Simplex Virus Type-2 Paralyzes the Function of Monocyte-Derived Dendritic Cells.
Grosche L, Mühl-Zürbes P, Ciblis B, Krawczyk A, Kuhnt C, Kamm L, Steinkasserer A, Heilingloh CS
Viruses 2020 Jan 16;12(1)
Viruses 2020 Jan 16;12(1)
Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.
Tong D, Zhang L, Ning F, Xu Y, Hu X, Shi Y
Protein & cell 2020 Feb;11(2):108-123
Protein & cell 2020 Feb;11(2):108-123
Endogenous Expression of the Human CD83 Attenuates EAE Symptoms in Humanized Transgenic Mice and Increases the Activity of Regulatory T Cells.
Zinser E, Naumann R, Wild AB, Michalski J, Deinzer A, Stich L, Kuhnt C, Steinkasserer A, Knippertz I
Frontiers in immunology 2019;10:1442
Frontiers in immunology 2019;10:1442
Role of Nuclear Factor (Erythroid-Derived 2)-Like 2 Signaling for Effects of Fumaric Acid Esters on Dendritic Cells.
Hammer A, Waschbisch A, Knippertz I, Zinser E, Berg J, Jörg S, Kuhbandner K, David C, Pi J, Bayas A, Lee DH, Haghikia A, Gold R, Steinkasserer A, Linker RA
Frontiers in immunology 2017;8:1922
Frontiers in immunology 2017;8:1922
ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.
Lecciso M, Ocadlikova D, Sangaletti S, Trabanelli S, De Marchi E, Orioli E, Pegoraro A, Portararo P, Jandus C, Bontadini A, Redavid A, Salvestrini V, Romero P, Colombo MP, Di Virgilio F, Cavo M, Adinolfi E, Curti A
Frontiers in immunology 2017;8:1918
Frontiers in immunology 2017;8:1918
In vitro and in vivo studies of the immunomodulatory effect of Echinacea purpurea on dendritic cells.
El-Ashmawy NE, El-Zamarany EA, Salem ML, El-Bahrawy HA, Al-Ashmawy GM
Journal, genetic engineering & biotechnology 2015 Dec;13(2):185-192
Journal, genetic engineering & biotechnology 2015 Dec;13(2):185-192
HIV-1 and HIV-2 differentially mature plasmacytoid dendritic cells into IFN-producing cells or APCs.
Royle CM, Graham DR, Sharma S, Fuchs D, Boasso A
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3538-48
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3538-48
Modulation of HIV-1-induced activation of plasmacytoid dendritic cells by 6-desfluoroquinolones.
Royle CM, Tsai MH, Tabarrini O, Massari S, Graham DR, Aquino VN, Boasso A
AIDS research and human retroviruses 2014 Apr;30(4):345-54
AIDS research and human retroviruses 2014 Apr;30(4):345-54
Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen.
Miles B, Scisci E, Carrion J, Sabino GJ, Genco CA, Cutler CW
Journal of leukocyte biology 2013 Aug;94(2):281-9
Journal of leukocyte biology 2013 Aug;94(2):281-9
Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling.
Moh MC, Lorenzini PA, Gullo C, Schwarz H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Aug;27(8):2957-66
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Aug;27(8):2957-66
Penicillium marneffei-stimulated dendritic cells enhance HIV-1 trans-infection and promote viral infection by activating primary CD4+ T cells.
Qin Y, Li Y, Liu W, Tian R, Guo Q, Li S, Li H, Zhang D, Zheng Y, Wu L, Lan K, Wang J
PloS one 2011;6(11):e27609
PloS one 2011;6(11):e27609
Immunological aspects of REIC/Dkk-3 in monocyte differentiation and tumor regression.
Watanabe M, Kashiwakura Y, Huang P, Ochiai K, Futami J, Li SA, Takaoka M, Nasu Y, Sakaguchi M, Huh NH, Kumon H
International journal of oncology 2009 Mar;34(3):657-63
International journal of oncology 2009 Mar;34(3):657-63
Decreased migration of Langerhans precursor-like cells in response to human keratinocytes expressing human papillomavirus type 16 E6/E7 is related to reduced macrophage inflammatory protein-3alpha production.
Guess JC, McCance DJ
Journal of virology 2005 Dec;79(23):14852-62
Journal of virology 2005 Dec;79(23):14852-62
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of 1-day PHA-stimulated normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or PE Anti-Human CD83 PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Identification of a gene expression signature linked to early dissemination. A Microarray time-series data analysis was performed on cells after treatment with BM-MSC-CM at 9 time points, from 0 to 96 h. Representative clusters of the indicated genes are shown as heatmaps, with red indicating increased expression and green indicating decreased expression, as indicated by the color intensity scale shown below each heatmap. B Functional fractionation of A549 cells via invasion assays. The percentage of cells expressing surface markers was determined by flow cytometry (CD133 + /CD83 + for early-response cells; CD151 + /CD38 + for late-response cells). C - E Functional fractionation of cancer cells via serial invasion assays. Cells were serially selected through 5 (HM5), 10 (HM10), or 20 (HM20) Matrigel invasion assays. The percentage of cells expressing surface markers was determined by QPCR (CD133 + /CD83 + in D ; CD151 + /CD38 + in E ). F QPCR showing the mRNA expression levels of STAT3 signaling-related genes. HM20 cells are compared to LM cells.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 PA T encounter with DCs results in limited activation involving upregulation of Il2 and Bcl2 . (A) PA T cells were co-cultured with DC1940 for 24 h, then CD4 + populations were gated into CD69 + and CD69 - cells by FACS. The expression of CD103, CXCR5, CD62L, CCR9, CCR7, CD127 and CD83 between CD69 + and CD69 - cells were compared. Three replicates in each group ( n = 3), and results are representative of three independent experiments ( N = 3). (B) CD69 + and CD69 - cells cultured in complete RPMI for 24 h without additional treatment, then the cytokines released to the supernatant were measured by ELISA. Pooled data from three independent experiments are shown. (C) Gene expression of Il7r , Il2 and Bcl2 was determined by QPCR in PA T cells or in CD69 positive or negative populations purified by FACS sorting. Three replicates in each group ( n = 3), and results are representative of three independent experiments ( N = 3). GAPDH was used for normalization
- Conjugate
- Yellow dye