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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
- Other assay [3]
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- Product number
- 17-0839-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD83 Monoclonal Antibody (HB15e), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HB15e monoclonal antibody reacts with human CD83, a 45 kDa transmembrane glycoprotein. CD83, a member of the Ig superfamily, is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells as well as some proliferating lymphocytes, and human cell lines express this antigen. While the function of CD83 is unclear, it can serve as a useful marker for mature human blood dendritic cells. Applications Reported: This HB15e antibody has been reported for use in flow cytometric analysis. Applications Tested: This HB15e antibody has been pre-titrated and tested by flow cytometric analysis of human dendritic cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HB15e
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references STAT3 phosphorylation at Ser727 and Tyr705 differentially regulates the EMT-MET switch and cancer metastasis.
Herpes Simplex Virus Type-2 Paralyzes the Function of Monocyte-Derived Dendritic Cells.
Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.
FcαRI co-stimulation converts human intestinal CD103(+) dendritic cells into pro-inflammatory cells through glycolytic reprogramming.
Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen.
Lin WH, Chang YW, Hong MX, Hsu TC, Lee KC, Lin C, Lee JL
Oncogene 2021 Jan;40(4):791-805
Oncogene 2021 Jan;40(4):791-805
Herpes Simplex Virus Type-2 Paralyzes the Function of Monocyte-Derived Dendritic Cells.
Grosche L, Mühl-Zürbes P, Ciblis B, Krawczyk A, Kuhnt C, Kamm L, Steinkasserer A, Heilingloh CS
Viruses 2020 Jan 16;12(1)
Viruses 2020 Jan 16;12(1)
Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.
Tong D, Zhang L, Ning F, Xu Y, Hu X, Shi Y
Protein & cell 2020 Feb;11(2):108-123
Protein & cell 2020 Feb;11(2):108-123
FcαRI co-stimulation converts human intestinal CD103(+) dendritic cells into pro-inflammatory cells through glycolytic reprogramming.
Hansen IS, Krabbendam L, Bernink JH, Loayza-Puch F, Hoepel W, van Burgsteden JA, Kuijper EC, Buskens CJ, Bemelman WA, Zaat SAJ, Agami R, Vidarsson G, van den Brink GR, de Jong EC, Wildenberg ME, Baeten DLP, Everts B, den Dunnen J
Nature communications 2018 Feb 28;9(1):863
Nature communications 2018 Feb 28;9(1):863
Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen.
Miles B, Scisci E, Carrion J, Sabino GJ, Genco CA, Cutler CW
Journal of leukocyte biology 2013 Aug;94(2):281-9
Journal of leukocyte biology 2013 Aug;94(2):281-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human monocytes were enriched and stimulated with Human GM-CSF, IL-4 and TNF alpha Recombinant Proteins for 7 days, then stained with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD83 APC (purple histogram). Cells in the large scatter population were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Identification of a gene expression signature linked to early dissemination. A Microarray time-series data analysis was performed on cells after treatment with BM-MSC-CM at 9 time points, from 0 to 96 h. Representative clusters of the indicated genes are shown as heatmaps, with red indicating increased expression and green indicating decreased expression, as indicated by the color intensity scale shown below each heatmap. B Functional fractionation of A549 cells via invasion assays. The percentage of cells expressing surface markers was determined by flow cytometry (CD133 + /CD83 + for early-response cells; CD151 + /CD38 + for late-response cells). C - E Functional fractionation of cancer cells via serial invasion assays. Cells were serially selected through 5 (HM5), 10 (HM10), or 20 (HM20) Matrigel invasion assays. The percentage of cells expressing surface markers was determined by QPCR (CD133 + /CD83 + in D ; CD151 + /CD38 + in E ). F QPCR showing the mRNA expression levels of STAT3 signaling-related genes. HM20 cells are compared to LM cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 PA T encounter with DCs results in limited activation involving upregulation of Il2 and Bcl2 . (A) PA T cells were co-cultured with DC1940 for 24 h, then CD4 + populations were gated into CD69 + and CD69 - cells by FACS. The expression of CD103, CXCR5, CD62L, CCR9, CCR7, CD127 and CD83 between CD69 + and CD69 - cells were compared. Three replicates in each group ( n = 3), and results are representative of three independent experiments ( N = 3). (B) CD69 + and CD69 - cells cultured in complete RPMI for 24 h without additional treatment, then the cytokines released to the supernatant were measured by ELISA. Pooled data from three independent experiments are shown. (C) Gene expression of Il7r , Il2 and Bcl2 was determined by QPCR in PA T cells or in CD69 positive or negative populations purified by FACS sorting. Three replicates in each group ( n = 3), and results are representative of three independent experiments ( N = 3). GAPDH was used for normalization
