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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00009308-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009308-M01, RRID:AB_537578
- Product name
- Mouse Monoclonal CD83 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CD83 - CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Zwitterionic polymer-coated immunobeads for blood-based cancer diagnostics.
Kim G, Yong Y, Kang HJ, Park K, Kim SI, Lee M, Huh N
Biomaterials 2014 Jan;35(1):294-303
Biomaterials 2014 Jan;35(1):294-303
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD83 Antibody (3G10-1F4) [H00009308-M01] - Analysis of CD83 expression in transfected 293T cell line by CD83 monoclonal antibody (M01), clone 3G10-1F4.Lane 1: CD83 transfected lysate (Predicted MW: 23 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD83 Antibody (3G10-1F4) [H00009308-M01] - CD83 monoclonal antibody (M01), clone 3G10-1F4. Analysis of CD83 expression in human colon.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CD83 Antibody (3G10-1F4) [H00009308-M01] - Detection limit for recombinant GST tagged CD83 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CD83 Antibody (3G10-1F4) [H00009308-M01] - Analysis of CD83 transfected lysate using anti-CD83 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CD83 MaxPab rabbit polyclonal antibody.
