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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Flow cytometry [2]
- Other assay [1]
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- Product number
- PA5-18389 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Parvalbumin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Tau Pathology Induces Excitatory Neuron Loss, Grid Cell Dysfunction, and Spatial Memory Deficits Reminiscent of Early Alzheimer's Disease.
Fu H, Rodriguez GA, Herman M, Emrani S, Nahmani E, Barrett G, Figueroa HY, Goldberg E, Hussaini SA, Duff KE
Neuron 2017 Feb 8;93(3):533-541.e5
Neuron 2017 Feb 8;93(3):533-541.e5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Brain lysate using Product # PA5-18389 at a concentration of 0.1 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PA5-18389 (0.15 µg/mL) in adenovirus-mediated gene transfer in rat heart cells 1) Untransfected, 2) transient transfection with Human PVALB. 3) Untransfected rat skeletal muscle, the primary antibody incubation was 1 hour and detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Parvalbumin by a Parvalbumin monoclonal antibody (Product # PA5-18389) at a concentration of 0.3 µg/mL. Human Cerebellum lysate (35µg protein in RIPA buffer).r. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Parvalbumin in Kelly cells using a Parvalbumin monoclonal antibody (Product # PA5-18389) at 10 µg/mL for 1hr, depicted by the blue line. The cells were paraformaldehyde fixed and permeabilized with 0.5% Triton. Primary incubation followed by Alexa Fluor 488 secondary antibody (2 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Parvalbumin in Kelly cells using a polyclonal antibody (Product #PA5-18389). Kelly cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (2 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
