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Western blotAntibody data
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- Western blot [1]
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- Product number
- BAF1786 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Serpin E1/PAI-1 Antibody
- Antibody type
- Polyclonal
Submitted references A multiplex immunoassay for human adipokine profiling.
Schipper HS, de Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clinical chemistry 2010 Aug;56(8):1320-8
Clinical chemistry 2010 Aug;56(8):1320-8
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Serpin E1/PAI-1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin E1/PAI-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1786) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. A specific band was detected for Serpin E1/PAI-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions.
