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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BAF3828 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Serpin E1/PAI-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Serpin E1/PAI-1 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Serpin E1 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) Serpin E2 and rmSerpin I1 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Mouse Serpin E1/PAI-1 by Western Blot. Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3828) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. A specific band was detected for Serpin E1/PAI-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions.
