MAB1786-100

antibody from Novus Biologicals

Targeting: SERPINE1 PAI, PAI1, PLANH1

Western blot (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1786-100

Provider

Novus Biologicals

Product name

Mouse Monoclonal Serpin E1/PAI-1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Serpin E1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Serpin A1, A3, A4, A8, C1, F1, F2, I1, I2, recombinant mouse Serpin D1 or E2 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Serpin E1/PAI-1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin E1/PAI-1 Monoclonal Antibody (Catalog # MAB1786) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin E1/PAI-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Serpin E1/PAI-1 by Simple Western^TM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI-1 at approximately 54 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human Serpin E1/PAI-1 Monoclonal Antibody (Catalog # MAB1786) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Neutralization of Serpin E1/ PAI-1 Activity by Human Serpin E1/PAI-1 Antibody. Recombinant Human u-Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, Catalog # 1310-SE) activity is measured in the presence of Recombinant Human Serpin E1/PAI-1 (0.25 µg/mL, Catalog # 1786-PI) that has been preincubated with increasing concentrations of Mouse Anti-Human Serpin E1/PAI-1 Monoclonal Antibody (Catalog # MAB1786). The ND₅₀ is typically 0.3 µg/mL.