MA5-23790

antibody from Invitrogen Antibodies

Targeting: SERPINE1 PAI, PAI1, PLANH1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Blocking/Neutralizing (Recommended by provider)

Antibody Data

Product number

MA5-23790

Provider

Invitrogen Antibodies

Product name

PAI1 Monoclonal Antibody (242816)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, no cross-reactivity with recombinant human Serpin A1, A3, A4, A8, C1, F1, F2, I1, I2, recombinant mouse Serpin D1 or E2 is observed. Reconstitute at 0.5 mg/mL in sterile PBS.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

242816

Vial size

500 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-PAI1 Monoclonal Antibody (242816) (Product # MA5-23790) and a 43 and 45 kDa band corresponding to PAI1 was observed across cell lines tested . Whole cell extracts (30 µg lysate) of HAEC (Lane 1), HUVEC (Lane 2), A549 (Lane 3), Raji (Lane 4), U-2 OS (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Both isoforms showed pick up as seen.