Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6774 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6774, RRID:AB_1580145
- Product name
- SERPINE1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of SERPINE1.
- Antigen sequence
C-GFKIDDKGMAPALRH
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Activation of mast cells by incorporation of cholesterol into rafts.
Baumruker T, Csonga R, Pursch E, Pfeffer A, Urtz N, Sutton S, Bofill-Cardona E, Cooke M, Prieschl E
International immunology 2003 Oct;15(10):1207-18
International immunology 2003 Oct;15(10):1207-18
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SERPINE1 polyclonal antibody (Cat # PAB6774) (0.1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SERPINE1 polyclonal antibody (Cat # PAB6774) (1.5 ug/mL) as the reporter with the capture rabbit antibody (2.5 ug/mL).
- Validation comment
- Sandwich ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SERPINE1 polyclonal antibody (Cat # PAB6774) (4 ug/mL) staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)