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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab120074 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- PAI1 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human PAI1 Antibody, Monoclonal(J11), could be used for WB, IHC, IF, ICC and so on . Application: WB: 0.01-2µg/mL IHC: 5-20µg/mL IF/ICC: 5-20µg/mL Protein Function This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 24-402- Antibody clone number
- J11
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- PAI1 Mouse mAb (Ab120074) - Western Blot All lanes: PAI1 Mouse mAb (Ab120074) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 45 kDa Observed band size: 47 kDa Exposure time: 20.5 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- PAI1 Mouse mAb (Ab120074) - IF/ICC FITC staining on IF Sample: Human HeLa cells Primary Ab: 20 μg/mL PAI1 Mouse mAb (Ab120074) Second Ab: 5 μg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- PAI1 Mouse mAb (Ab120074) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 20 μg/mL PAI1 Mouse mAb (Ab120074) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
