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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- NB300-212 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-212, RRID:AB_10002231
- Product name
- Rabbit Polyclonal Tyrosine Hydroxylase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser19
- Reactivity
- Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Tyrosine Hydroxylase [p Ser19] Antibody [NB300-212] - Performed on three different samples. Lane A: non-phospho recombinant TH. Lane B: PC-12 cell lysate. Lane C: PC-12 cell lysate from cells stimulation for 10 min with 30 mM KCl.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Tyrosine Hydroxylase [p Ser19] Antibody [NB300-212] - Non-phospho-recombinant tyrosine hydroxylase (Lane 1), PC-12 cell lysate (Lane 2), and PC-12 cell lysate stimulated with KCl, 30 mM for 10 min (Lane 3) showing specific immunolabeling of the ~60k tyrosine hydroxylase phosphorylated at Ser19.
