P21962

antibody from Invitrogen Antibodies

Targeting: TH DYT5b

Provider product page for P21962

Western blot

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [9]
Comments [0]
Validations
Western blot [2]
Immunohistochemistry [1]
Other assay [2]

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Product number: P21962 - Provider product page
Provider: Invitrogen Antibodies
Product name: Tyrosine Hydroxylase Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Purifed from natural sources
Reactivity: Human, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Storage: -20° C, Avoid Freeze/Thaw Cycles

TH protein structure - P21962 shown in red.

Submitted references Insulin Bidirectionally Alters NAc Glutamatergic Transmission: Interactions between Insulin Receptor Activation, Endogenous Opioids, and Glutamate Release.

Fetterly TL, Oginsky MF, Nieto AM, Alonso-Caraballo Y, Santana-Rodriguez Z, Ferrario CR
The Journal of neuroscience : the official journal of the Society for Neuroscience 2021 Mar 17;41(11):2360-2372

Absence of Glia Maturation Factor Protects from Axonal Injury and Motor Behavioral Impairments after Traumatic Brain Injury.

Selvakumar GP, Ahmed ME, Iyer SS, Thangavel R, Kempuraj D, Raikwar SP, Bazley K, Wu K, Khan A, Kukulka K, Bussinger B, Zaheer S, Burton C, James D, Zaheer A
Experimental neurobiology 2020 Jun 30;29(3):230-248

Ventral Tegmental Dopamine Neurons Control the Impulse Vector during Motivated Behavior.

Hughes RN, Bakhurin KI, Petter EA, Watson GDR, Kim N, Friedman AD, Yin HH
Current biology : CB 2020 Jul 20;30(14):2681-2694.e5

Dexmedetomidine Activation of Dopamine Neurons in the Ventral Tegmental Area Attenuates the Depth of Sedation in Mice.

Qiu G, Wu Y, Yang Z, Li L, Zhu X, Wang Y, Sun W, Dong H, Li Y, Hu J
Anesthesiology 2020 Aug;133(2):377-392

Psychological Stress Phenocopies Brain Mitochondrial Dysfunction and Motor Deficits as Observed in a Parkinsonian Rat Model.

Grigoruţă M, Martínez-Martínez A, Dagda RY, Dagda RK
Molecular neurobiology 2020 Apr;57(4):1781-1798

Synaptic Organization of VGLUT3 Expressing Low-Threshold Mechanosensitive C Fiber Terminals in the Rodent Spinal Cord.

Larsson M, Broman J
eNeuro 2019 Jan-Feb;6(1)

Wakefulness Is Promoted during Day Time by PDFR Signalling to Dopaminergic Neurons in Drosophila melanogaster.

Potdar S, Sheeba V
eNeuro 2018 Jul-Aug;5(4)

The Neuroregenerative Capacity of Olfactory Stem Cells Is Not Limitless: Implications for Aging.

Child KM, Herrick DB, Schwob JE, Holbrook EH, Jang W
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Aug 1;38(31):6806-6824

Role of the IL-1 Pathway in Dopaminergic Neurodegeneration and Decreased Voluntary Movement.

Stojakovic A, Paz-Filho G, Arcos-Burgos M, Licinio J, Wong ML, Mastronardi CA
Molecular neurobiology 2017 Aug;54(6):4486-4495

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of 10 µg of rat caudate lysate showing specific immunolabeling of the ~60 kDa TH protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of 10 µg of rat caudate lysate showing specific immunolabeling of the ~60 kDa TH protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of DA neurons using anti-tyrosine hydroxylase (TH) antibody. H9 ESCs were differentiated with PSC Dopaminergic neuron differentiation kit. H9 ESCs were specified to become midbrain floor plate (FP) progenitors which were further expanded and cryopreserved. Recovered FP progenitors were then matured for additional 14 days. Expression of TH was labeled with 1st antibody, anti-TH (Product # P21962) followed by 2nd antibody AlexaFluor488 goat anti-rabbit (Product # A-21206, green). Nuclear DNA was stained with DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6. High-fat diet-induced obesity results in a loss of insulin-induced increases in excitatory transmission and a reduction in NAc IRbeta surface expression. Average concentration of fasted plasma insulin ( A ) and fat mass ( B ) in chow and high-fat diet groups. C , Average eEPSC amplitude following bath application of increasing concentrations of insulin (blue bars) and following insulin washout in MSNs from chow (circles) and high-fat groups (squares). Right, Representative traces for each group before (black) and after (blue) each insulin concentration. D , Average NAc IRbeta surface expression in high-fat and chow-fed groups. Left, immunoblot for TH in the bound ( B ) and unbound (UB) fractions. Consistent with its intracellular localization, TH protein levels were nearly absent in the bound (surface) fraction. E , Total NAc IRbeta expression in high-fat and chow-fed groups. Representative blot images are shown below each graph. Statistical differences were determined by two-tailed unpaired t tests ( A , B : ** p < 0.001), two-way repeated-measures ANOVA comparing BL and treatment conditions ( C : *chow group, main effect of treatment; # high-fat group, main effect of treatment, p < 0.05), and two-tailed unpaired t test ( D : p = 0.046).