Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-42211AF647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Tyrosine Hydroxylase Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Tyrosine Hydroxylase Antibody (5C7.2E8) [Alexa Fluor (R) 647] [NBP2-42211AF647] - An intracellular stain was performed on SH-SY5Y cells with Tyrosine Hydroxylase (5C7.2E8) antibody NBP2-42211AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.