Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [3]
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Validation data
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- Product number
- NBP2-42211 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Tyrosine Hydroxylase Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Apelin-36 mediates neuroprotective effects by regulating oxidative stress, autophagy and apoptosis in MPTP-induced Parkinson's disease model mice.
Zhu J, Gao W, Shan X, Wang C, Wang H, Shao Z, Dou S, Jiang Y, Wang C, Cheng B
Brain research 2020 Jan 1;1726:146493
Brain research 2020 Jan 1;1726:146493
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Tyrosine Hydroxylase Antibody (5C7.2E8) [NBP2-42211] - Western blot analysis of a partial recombinant human Tyrosine Hydroxylase protein and a lysate of HEK293 cells using 3 ug/mL of Tyrosine Hydroxylase antibody clone 5C7.2E8. The antibody detected ~11 kDa and ~58 kDa specific bands representing the recombinant and the endogenous TH proteins respectively.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Tyrosine Hydroxylase Antibody (5C7.2E8) [NBP2-42211] - Immunohistochemical analysis of a FFPE tissue section of human brain/substantia nigra using Tyrosine Hydroxylase antibody (clone 5C7.2E8) at 15 ug/mL. This representative photomicrograph shows strong immunostaining of tyrosine hydroxylase/TH in TH-positive neurons.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): Tyrosine Hydroxylase Antibody (5C7.2E8) [NBP2-42211] - An intracellular stain was performed on SH-SY5Y cells with Tyrosine Hydroxylase (5C7.2E8) antibody NBP2-42211 (blue) and a matched isotype control NBP2-14864 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (F0102B, R&D systems).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Tyrosine Hydroxylase Antibody (5C7.2E8) [NBP2-42211] - An intracellular stain was performed on SH-SY5Y cells with Tyrosine Hydroxylase (5C7.2E8) antibody NBP2-42211AF488 (blue) and a matched isotype control NB600-986AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Tyrosine Hydroxylase Antibody (5C7.2E8) [NBP2-42211] - Analysis of Alexa Fluor (R) 647 conjugate of NBP2-42211. An intracellular stain was performed on SH-SY5Y cells with Tyrosine Hydroxylase (5C7.2E8) antibody NBP2-42211AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with