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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [4]
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Validation data
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- Product number
- TA506542 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TH Monoclonal Antibody (OTI3H3), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI3H3
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TH (RC211218, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TH. Positive lysates LY424777 (100 µg) and LC424777 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-TH monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-TH mouse monoclonal antibody (TA506542) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TH(RC211218).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-TH mouse monoclonal antibody (TA506542).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-TH mouse monoclonal antibody (TA506542) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TH(RC211218).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-TH mouse monoclonal antibody (TA506542).
