NB300-173

antibody from Novus Biologicals

Targeting: TH DYT5b

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-173

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-173, RRID:AB_2201648

Product name

Rabbit Polyclonal Tyrosine Hydroxylase Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specific for the ~60 kDa tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.

Reactivity

Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Acrolein-mediated alpha-synuclein pathology involvement in the early post-injury pathogenesis of mild blast-induced Parkinsonian neurodegeneration.

Acosta G, Race N, Herr S, Fernandez J, Tang J, Rogers E, Shi R
Molecular and cellular neurosciences 2019 Jul;98:140-154

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Impaired ventilatory acclimatization to hypoxia in female mice overexpressing erythropoietin: unexpected deleterious effect of estradiol in carotid bodies.

Gassmann M, Pfistner C, Doan VD, Vogel J, Soliz J
American journal of physiology. Regulatory, integrative and comparative physiology 2010 Dec;299(6):R1511-20

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Erythropoietin regulates hypoxic ventilation in mice by interacting with brainstem and carotid bodies.

Soliz J, Joseph V, Soulage C, Becskei C, Vogel J, Pequignot JM, Ogunshola O, Gassmann M
The Journal of physiology 2005 Oct 15;568(Pt 2):559-71

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Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina.

Witkovsky P, Gabriel R, Haycock JW, Meller E
Journal of chemical neuroanatomy 2000 Jun;19(2):105-16

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Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina.

Witkovsky P, Gabriel R, Haycock JW, Meller E
Journal of chemical neuroanatomy 2000 Jun;19(2):105-16

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Stoichiometry of tyrosine hydroxylase phosphorylation in the nigrostriatal and mesolimbic systems in vivo: effects of acute haloperidol and related compounds.

Salvatore MF, Garcia-Espana A, Goldstein M, Deutch AY, Haycock JW
Journal of neurochemistry 2000 Jul;75(1):225-32

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Stoichiometry of tyrosine hydroxylase phosphorylation in the nigrostriatal and mesolimbic systems in vivo: effects of acute haloperidol and related compounds.

Salvatore MF, Garcia-Espana A, Goldstein M, Deutch AY, Haycock JW
Journal of neurochemistry 2000 Jul;75(1):225-32

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Increased site-specific phosphorylation of tyrosine hydroxylase accompanies stimulation of enzymatic activity induced by cessation of dopamine neuronal activity.

Lew JY, Garcia-Espana A, Lee KY, Carr KD, Goldstein M, Haycock JW, Meller E
Molecular pharmacology 1999 Feb;55(2):202-9

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Tyrosine Hydroxylase [p Ser40] Antibody [NB300-173] - Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and phospho-specific tyrosine hydroxylase (phospho-TH) antibodies. The pan-TH antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the phospho-TH antibody selectively labels only the two amacrine cells in this light-stimulated retina example.