44-1109G

antibody from Invitrogen Antibodies

Targeting: ETS1 ETS-1, EWSR2, FLJ10768

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

44-1109G

Provider

Invitrogen Antibodies

Product name

Phospho-ETS1 (Ser282) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purity: Greater than 90%, as determined by SDS-PAGE.

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20°C

References

Submitted references

Phosphorylation of ETS1 by Src family kinases prevents its recognition by the COP1 tumor suppressor.

Lu G, Zhang Q, Huang Y, Song J, Tomaino R, Ehrenberger T, Lim E, Liu W, Bronson RT, Bowden M, Brock J, Krop IE, Dillon DA, Gygi SP, Mills GB, Richardson AL, Signoretti S, Yaffe MB, Kaelin WG Jr
Cancer cell 2014 Aug 11;26(2):222-34

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lysates from Jurkat cells either untreated (1, 6), treated with hydrogen peroxide (2-5), or ionophore A23187 (7) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% milk-TBST buffer for one hour at room temperature, then were incubated with the ETS1 (pS 282) antibody overnight at 4%deg;C in a 1% milk-TBST buffer, following prior incubation with: no peptide (1-2, 6-7), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F (ab′)2 anti-rabbit IgG HRP conjugate (Product # ALI4404), and bands were detected using the Pierce SuperSignal™ method.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lysates from Jurkat cells either untreated (1, 6), treated with hydrogen peroxide (2-5), or ionophore A23187 (7) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% milk-TBST buffer for one hour at room temperature, then were incubated with the ETS1 (pS 282) antibody overnight at 4%deg;C in a 1% milk-TBST buffer, following prior incubation with: no peptide (1-2, 6-7), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F (ab′)2 anti-rabbit IgG HRP conjugate (Product # ALI4404), and bands were detected using the Pierce SuperSignal™ method.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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