Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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Validation data
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- Product number
- 44-1109G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-ETS1 (Ser282) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purity: Greater than 90%, as determined by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Phosphorylation of ETS1 by Src family kinases prevents its recognition by the COP1 tumor suppressor.
Lu G, Zhang Q, Huang Y, Song J, Tomaino R, Ehrenberger T, Lim E, Liu W, Bronson RT, Bowden M, Brock J, Krop IE, Dillon DA, Gygi SP, Mills GB, Richardson AL, Signoretti S, Yaffe MB, Kaelin WG Jr
Cancer cell 2014 Aug 11;26(2):222-34
Cancer cell 2014 Aug 11;26(2):222-34
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lysates from Jurkat cells either untreated (1, 6), treated with hydrogen peroxide (2-5), or ionophore A23187 (7) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% milk-TBST buffer for one hour at room temperature, then were incubated with the ETS1 (pS 282) antibody overnight at 4%deg;C in a 1% milk-TBST buffer, following prior incubation with: no peptide (1-2, 6-7), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F (ab′)2 anti-rabbit IgG HRP conjugate (Product # ALI4404), and bands were detected using the Pierce SuperSignal™ method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lysates from Jurkat cells either untreated (1, 6), treated with hydrogen peroxide (2-5), or ionophore A23187 (7) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% milk-TBST buffer for one hour at room temperature, then were incubated with the ETS1 (pS 282) antibody overnight at 4%deg;C in a 1% milk-TBST buffer, following prior incubation with: no peptide (1-2, 6-7), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F (ab′)2 anti-rabbit IgG HRP conjugate (Product # ALI4404), and bands were detected using the Pierce SuperSignal™ method.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL