Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 500-P97-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TARC Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQGRAIC SDPNNKRVKN AVKYLQSLER S. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TARC (CCL17). Anti-Human TARC (CCL17)-specific antibody was purified by affinity chromatography employing an immobilized Human TARC (CCL17) matrix. Sandwich ELISA: To detect Human TARC (CCL17) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TARC (CCL17) (500-P97Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TARC (CCL17). Western Blot: To detect hTARC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTARC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Cross talk between follicular Th cells and tumor cells in human follicular lymphoma promotes immune evasion in the tumor microenvironment.
Dendritic cells exposed to extracellular adenosine triphosphate acquire the migratory properties of mature cells and show a reduced capacity to attract type 1 T lymphocytes.
Rawal S, Chu F, Zhang M, Park HJ, Nattamai D, Kannan S, Sharma R, Delgado D, Chou T, Lin HY, Baladandayuthapani V, Luong A, Vega F, Fowler N, Dong C, Davis RE, Neelapu SS
Journal of immunology (Baltimore, Md. : 1950) 2013 Jun 15;190(12):6681-93
Journal of immunology (Baltimore, Md. : 1950) 2013 Jun 15;190(12):6681-93
Dendritic cells exposed to extracellular adenosine triphosphate acquire the migratory properties of mature cells and show a reduced capacity to attract type 1 T lymphocytes.
la Sala A, Sebastiani S, Ferrari D, Di Virgilio F, Idzko M, Norgauer J, Girolomoni G
Blood 2002 Mar 1;99(5):1715-22
Blood 2002 Mar 1;99(5):1715-22
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human TARC (CCL17) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of TARC Polyclonal Antibody (Product # 500-P97-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech TARC Polyclonal Antibody, Biotin (Product # 500-P97BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TARC (CCL17).
