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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- IC3641P - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL17/TARC PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CCL17/TARC in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27 or recombinant rat CCL20 is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Q92583.1- Isotype
- IgG
- Antibody clone number
- 54015
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references CCL17 and CCL22 chemokines within tumor microenvironment are related to accumulation of Foxp3+ regulatory T cells in gastric cancer.
SHP-1 deficiency and increased inflammatory gene expression in PBMCs of multiple sclerosis patients.
Mizukami Y, Kono K, Kawaguchi Y, Akaike H, Kamimura K, Sugai H, Fujii H
International journal of cancer 2008 May 15;122(10):2286-93
International journal of cancer 2008 May 15;122(10):2286-93
SHP-1 deficiency and increased inflammatory gene expression in PBMCs of multiple sclerosis patients.
Christophi GP, Hudson CA, Gruber RC, Christophi CP, Mihai C, Mejico LJ, Jubelt B, Massa PT
Laboratory investigation; a journal of technical methods and pathology 2008 Mar;88(3):243-55
Laboratory investigation; a journal of technical methods and pathology 2008 Mar;88(3):243-55
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of CCL17/TARC in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human CCL17/TARC PE-conjugated Monoclonal Antibody (Catalog # IC3641P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
