Antibody data
- Product number
- MA5-14919
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Torc3 Monoclonal Antibody (R.185.4)
- Provider product page
- Invitrogen Antibodies - MA5-14919
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- R.185.4
- Vial size
- 100 µL
- Concentration
- Lot Dependent
- Storage
- -20°C
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Torc3 in extracts from MCF-7 and LN18 cells using Torc3 monoclonal antibody (Product # MA5-14919).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Torc3 was achieved by transfecting U-2 OS cells with Torc3 specific siRNAs (Silencer® select Product # s34938, s34939). Western blot analysis (Fig. a) was performed using whole cell extracts from the Torc3 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Torc3 Monoclonal Antibody (R.185.4) (Product # MA5-14919, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Torc3.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hela (Lane 1), A431 (Lane 2) U2OS (Lane 3), HEK293 (Lane 4), Jurkat (Lane 5), COS-7 (Lane 6) and MCF-7 (Lane 7). The blot was probed with Anti-Torc3 Monoclonal Antibody (Product # MA5-14919, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 67 kDa band corresponding to Torc3 was observed in HeLa, U2OS and HEK293 when compared to other cell lines which are reported to have low levels of Torc3 expression.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Torc3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-Torc3 Rabbit Monoclonal Antibody (Product # MA5-14919) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive data in Antibodypedia
Data presented on provider website
