R20185

antibody from NSJ Bioreagents

Targeting: IGHD

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R20185

Provider

NSJ Bioreagents

Product name

Recombinant Human IgD Antibody

Antibody type

Monoclonal

Description

This highly specific Recombinant Human IgD antibody is suitable for use in ELISA applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

RM123

Vial size

100 ug

Concentration

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Storage

Store the recombinant Human IgD antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

ELISA

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Sandwich ELISA of human plasma using recombinant Human IgD antibody as the capture (100ng/well) and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ELISA of hIgs shows recombinant Human IgD antibody reacts to IgD from human plasma and IgD,κ from human myeloma. No cross reactivity with IgG, IgM, IgA, or IgE.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ELISA Titration: the plate was coated with different amounts of human IgD (from plasma). A serial dilution of recombinant Human IgD antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human lymphoid tissue with recombinant Human IgD antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.