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- Antibody Data
- Antigen structure
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- Validations
- ELISA [3]
- Immunohistochemistry [1]
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- Product number
- R20185BTN - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Human IgD Antibody (Biotin Conjugate)
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant Human IgD antibody is suitable for use in ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM123
- Vial size
- 50 ug
- Concentration
- 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant Human IgD antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA of human plasma using recombinant Human IgD antibody as the capture (100ng/well) and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA of hIgs shows recombinant Human IgD antibody reacts to IgD from human plasma and IgD,κ from human myeloma. No cross reactivity with IgG, IgM, IgA, or IgE.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA Titration: the plate was coated with different amounts of human IgD (from plasma). A serial dilution of recombinant Human IgD antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lymphoid tissue with recombinant Human IgD antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
