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antibody from GeneTex
Targeting: CDKN1A
CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
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- Product number
- GTX629543 - Provider product page
- Provider
- GeneTex
- Product name
- p21 Cip1 antibody [GT1032]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
Submitted references Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Preprogramming therapeutic response of PI3K/mTOR dual inhibitor via the regulation of EHMT2 and p27 in pancreatic cancer.
Hinokitiol Negatively Regulates Immune Responses through Cell Cycle Arrest in Concanavalin A-Activated Lymphocytes.
High glucose induces bone marrow-derived mesenchymal stem cell senescence by upregulating autophagy.
Li HY, Leu YL, Wu YC, Wang SH
Journal of agricultural and food chemistry 2019 Feb 20;67(7):1889-1901
Journal of agricultural and food chemistry 2019 Feb 20;67(7):1889-1901
Preprogramming therapeutic response of PI3K/mTOR dual inhibitor via the regulation of EHMT2 and p27 in pancreatic cancer.
Tian YF, Wang HC, Luo CW, Hung WC, Lin YH, Chen TY, Li CF, Lin CY, Pan MR
American journal of cancer research 2018;8(9):1812-1822
American journal of cancer research 2018;8(9):1812-1822
Hinokitiol Negatively Regulates Immune Responses through Cell Cycle Arrest in Concanavalin A-Activated Lymphocytes.
Chung CL, Leung KW, Lu WJ, Yen TL, He CF, Sheu JR, Lin KH, Lien LM
Evidence-based complementary and alternative medicine : eCAM 2015;2015:595824
Evidence-based complementary and alternative medicine : eCAM 2015;2015:595824
High glucose induces bone marrow-derived mesenchymal stem cell senescence by upregulating autophagy.
Chang TC, Hsu MF, Wu KK
PloS one 2015;10(5):e0126537
PloS one 2015;10(5):e0126537
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- p21 Cip1 antibody [GT1032] detects p21 Cip1 protein by western blot analysis.A. 30 ?g HCT116 whole cell lysate/extract (untreated)B. 30 ?g HCT116 whole cell lysate/extract (30 ?M Cisplatin treatment for 24 hr)C. 30 ?g HCT116 whole cell lysate/extract (30 ?M Cisplatin treatment for 48 hr)15% SDS-PAGEp21 Cip1 antibody [GT1032] (GTX629543) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.Sample: HCT116 cells treated with 30£gM cisplatin for 24hr and were fixed in 4% paraformaldehyde at RT for 15 min.Green: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody (GTX102993) diluted at 1:1000.Red: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- p21 Cip1 antibody [GT1032] immunoprecipitates CDKN1A protein in IP experiments.IP samples: HCT-116 whole cell extract treat with 30uM cisplatin for 48 hrA. 30 £gg HCT-116 whole cell extract treat with 30uM cisplatin for 48 hrB. Control with 4 £gg of preimmune Mouse IgGC. Immunoprecipitation of CDKN1A protein by 4 £gg p21 Cip1 antibody [GT1032] (GTX629543)15 % SDS-PAGEThe immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:1000.[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]
