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LearnGTX629627
antibody from GeneTex
Targeting: CDKN1A
CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- GTX629627 - Provider product page
- Provider
- GeneTex
- Product name
- p21 Cip1 antibody [GT8611]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- p21 Cip1 antibody [GT8611] detects p21 Cip1 protein by Western blot analysis.A. 30 ?g HCT116 whole cell lysate/extract (untreated)B. 30 ?g HCT116 whole cell lysate/extract (30 ?M Cisplatin treatment for 24 hr)15 % SDS-PAGEp21 Cip1 antibody [GT8611] (GTX629627) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT8611] (GTX629627) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT8611] (GTX629627) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- p21 Cip1 antibody [GT8611] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.Sample: HCT116 cells treated with 30£gM cisplatin for 24hr and were fixed in 4% paraformaldehyde at RT for 15 min.Green: p21 Cip1 protein stained by p21 Cip1 antibody [GT8611] (GTX629627) diluted at 1:500.Blue: Hoechst 33342 staining.
