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antibody from Invitrogen Antibodies
Targeting: CDKN1A
CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-34805 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p21 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HCT116 treated 30uM cisplatin 24hr. Predicted reactivity: Cat (84%), Sheep (82%), Rhesus Monkey (98%), Bovine (83%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p21/Cip1 using 30 µg of A) HCT116 cells with 30uM cisplatin treatment for 0hr and B) HCT116 cells with 30uM cisplatin treatment for 24hr lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a p21/Cip1 polyclonal antibody (Product # PA5-34805) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of p21 was performed by separating various whole cell extracts by 12% SDS PAGE. Proteins were transferred to a membrane and probed with a p21 Polyclonal Antibody (Product # PA5-34805) at a dilution of 1:1000. A: HCT116, B: HCT116 treated with 30uM cisplatin for 24hr.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of p21/Cip1 showing staining in the nucleus of HCT116 cells. HCT116 cells mock and treated with 30µM cisplatin for 24hr and were fixed in 4% paraformaldehyde at RT for 15 min and stained using a p21/Cip1 polyclonal antibody (Product # PA5-34805) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of p21 using p21 Polyclonal Antibody (Product # PA5-34805) p21 Cip1 antibody detects p21 Cip1 protein at nucleus by immunofluorescent analysis. Samples used were HeLa cells mock (left) and HeLa cells treated with 30 µM Cisplatin for 24 hrs (right) fixed with 4% paraformaldehyde at RT for 15 min. Green: p21 Polyclonal Antibody (Product # PA5-34805) diluted at 1:500. Blue: Hoechst 33342 staining Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- p21 Polyclonal Antibody detects CDKN1A protein at nucleus on SkHep1xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SkHep1xenograft. P21 Polyclonal Antibody (Product # PA5-34805) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- p21 Polyclonal Antibody detects p21 Cip1 protein at nucleus by immunohistochemical analysis (Autostainer Formulated). Sample: Paraffin-embedded human normal colon tissue. p21 Cip1 stained by p21 Polyclonal Antibody (Product # PA5-34805) diluted at 1:500. Antigen Retrieval: EDTA buffer, 20 min.
