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antibody from Invitrogen Antibodies
Targeting: CDKN1A
CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-37519 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-p21 (Thr145) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for IHC is human breast carcinoma.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references The novel roles of virus infection-associated gene CDKN1A in chemoresistance and immune infiltration of glioblastoma.
Hu K, Li J, Wu G, Zhou L, Wang X, Yan Y, Xu Z
Aging 2021 Feb 17;13(5):6662-6680
Aging 2021 Feb 17;13(5):6662-6680
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-p21 (Thr145) in paraffin-embedded Human breast carcinoma tissue using Phospho-p21 (Thr145) Polyclonal Antibody (Product # PA5-37519) (left) or the same antibody preincubated with blocking peptide (right).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 CDKN1A was involved in TMZ resistance of glioma cells. ( A ) Western blot for phospho-Akt (Ser-473), Akt in U87, U87-R T98G, and T98G-R cells. ( B ) Western blot for phospho-Akt (Ser-473), Akt, phospho-CDKN1A, and CDKN1A in U87-R or T98G-R cells treated with DMSO and the specific inhibitor to AKT MK2206. ( C ) Western blot for phospho-Akt (Ser-473), Akt, phospho-CDKN1A, and CDKN1A in U87-R or T98G-R cells treated with siCtrl and siCDKN1A. ( D ) Western blot for phospho-Akt (Ser-473), Akt, phospho-CDKN1A, and CDKN1A in U87-R cells treated with DMSO, MK2206, siCDKN1A and MK2206 plus siCDKN1A. ( E ) Western blot for phospho-Akt (Ser-473), Akt, phospho-CDKN1A, and CDKN1A in T98G-R cells treated with DMSO, MK2206, siCDKN1A and MK2206 plus siCDKN1A. ( F , G ) Colony formation assay of U87-R or T98G-R cells treated with DMSO, MK2206, siCDKN1A and MK2206 plus siCDKN1A. The results were presented as means +- SD (n = 3 for each panel). Statistical significance was concluded at *P < 0.05, **P < 0.01.
