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LearnPAB0556
antibody from Abnova Corporation
Targeting: CDKN1A
CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PAB0556 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0556, RRID:AB_982766
- Product name
- CDKN1A (phospho T57) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDKN1A.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Interplay between HIV-1 Vpr and Sp1 modulates p21(WAF1) gene expression in human astrocytes.
Clusterin-mediated apoptosis is regulated by adenomatous polyposis coli and is p21 dependent but p53 independent.
Methylation status of cyclin-dependent kinase inhibitor genes within the transforming growth factor beta pathway in human T-cell lymphoblastic lymphoma/leukemia.
Amini S, Saunders M, Kelley K, Khalili K, Sawaya BE
The Journal of biological chemistry 2004 Oct 29;279(44):46046-56
The Journal of biological chemistry 2004 Oct 29;279(44):46046-56
Clusterin-mediated apoptosis is regulated by adenomatous polyposis coli and is p21 dependent but p53 independent.
Chen T, Turner J, McCarthy S, Scaltriti M, Bettuzzi S, Yeatman TJ
Cancer research 2004 Oct 15;64(20):7412-9
Cancer research 2004 Oct 15;64(20):7412-9
Methylation status of cyclin-dependent kinase inhibitor genes within the transforming growth factor beta pathway in human T-cell lymphoblastic lymphoma/leukemia.
Scott SA, Kimura T, Dong WF, Ichinohasama R, Bergen S, Kerviche A, Sheridan D, DeCoteau JF
Leukemia research 2004 Dec;28(12):1293-301
Leukemia research 2004 Dec;28(12):1293-301
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CDKN1A (phospho T57) polyclonal antibody (Cat # PAB0556). Western blot analysis of CDKN1A expression in human colon.
- Validation comment
- Western Blot (Tissue lysate)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CDKN1A (phospho T57) polyclonal antibody (Cat # PAB0556) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of CDKN1A (phospho T57) polyclonal antibody (Cat # PAB0556) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
