M00145-3

antibody from Boster Biological Technology

Targeting: CDKN1A CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00145-3

Provider

Boster Biological Technology

Product name

Anti-p21 CDKN1A Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for p21/CDKN1A detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IP, IF, IHC, ICC, WB. Reactive species: Human p21/CDKN1A information: Molecular Weight: 18119 MW; Subcellular Localization: Cytoplasm. Nucleus; Tissue Specificity: Expressed in all adult tissues, with 5-fold lower levels observed in the brain.

Reactivity

Human

Host

Rabbit

Antibody clone number

ACG-3

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

PHLDA2 overexpression facilitates senescence and apoptosis via the mitochondrial route in human nucleus pulposus cells by regulating Wnt/β-catenin signalling pathway.

Chang X, Cao Y, Hu ZL, Zhai Y, Zhang YY, Lv YF, Li CQ
IUBMB life 2024 May 9;

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Vitamin C Inhibited Pulmonary Metastasis through Activating Nrf2/HO-1 Pathway.

Man S, Bi J, Liu F, Xie W, Ma L
Molecular nutrition & food research 2024 Mar;68(6):e2300706

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p21 Prevents the Exhaustion of CD4(+) T Cells Within the Antitumor Immune Response Against Colorectal Cancer.

Thoma OM, Naschberger E, Kubánková M, Larafa I, Kramer V, Menchicchi B, Merkel S, Britzen-Laurent N, Jefremow A, Grützmann R, Koop K, Neufert C, Atreya R, Guck J, Stürzl M, Neurath MF, Waldner MJ
Gastroenterology 2024 Feb;166(2):284-297.e11

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Synthesis, biological activity and mechanism of action of novel allosecurinine derivatives as potential antitumor agents.

Xu XL, Lan JX, Huang H, Dai W, Peng XP, Liu SL, Chen WM, Huang LJ, Liu J, Li XJ, Zeng JL, Huang XH, Zhao GN, Hou W
Bioorganic & medicinal chemistry 2023 Mar 15;82:117234

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Angelica sinensis polysaccharides alleviate the oxidative burden on hematopoietic cells by restoring 5-fluorouracil-induced oxidative damage in perivascular mesenchymal progenitor cells.

Niu Y, Xiao H, Wang B, Wang Z, Du K, Wang Y, Wang L
Pharmaceutical biology 2023 Dec;61(1):768-778

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Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration.

Chen Q, Fu L, Hu J, Guo G, Xie A
Cancer cell international 2021 Apr 26;21(1):235

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Leucine aminopeptidase 3 promotes migration and invasion of breast cancer cells through upregulation of fascin and matrix metalloproteinases-2/9 expression.

Fang C, Zhang J, Yang H, Peng L, Wang K, Wang Y, Zhao X, Liu H, Dou C, Shi L, Zhao C, Liang S, Li D, Wang X
Journal of cellular biochemistry 2019 Mar;120(3):3611-3620

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon, using p21 Antibody(M00145-3)CDKN1A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDKN1A Antibody (M00145-3)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image