60214-1-Ig

antibody from Proteintech Group

Targeting: CDKN1A CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1

Provider product page for 60214-1-Ig

Western blot

ELISA

Immunocytochemistry

Antibody data

Antibody Data
Antigen structure
References [29]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 60214-1-Ig - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#60214-1-Ig, RRID:AB_11042450
Product name: P21 antibody
Antibody type: Monoclonal
Description: KD/KO validated P21 antibody (Cat. #60214-1-Ig) is a mouse monoclonal antibody that shows reactivity with monkey and has been validated for the following applications: ELISA.
Reactivity: Simian
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 5F2F3
Vial size: 20ul, 150ul

CDKN1A protein structure - 60214-1-Ig shown in red.

Submitted references Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer.

Wang R, Mi Y, Ni J, Wang Y, Ding L, Ran X, Sun Q, Tan SY, Koeffler HP, Feng N, Chen YQ
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Mar;11(9):e2304939

Increased soluble E‑cadherin of spheroid formation supplemented with fetal bovine serum in colorectal cancer cells.

Chang IY, Yoon SP
Oncology letters 2023 May;25(5):207

Erxian decoction alleviates cisplatin-induced premature ovarian failure in rats by reducing oxidation levels in ovarian granulosa cells.

Liu J, Yang Y, He Y, Feng C, Ou H, Yang J, Chen Y, You F, Shao B, Bao J, Guan X, Chen F, Zhao P
Journal of ethnopharmacology 2023 Mar 25;304:116046

Bovine milk exosomes affected proliferation of macrophages under hypoxia.

Matic S, Dia VP
Current research in food science 2022;5:2108-2113

Depletion of PARP10 inhibits the growth and metastatic potential of oral squamous cell carcinoma.

Zhou Z, Wei B, Liu Y, Liu T, Zeng S, Gan J, Qi G
Frontiers in genetics 2022;13:1035638

N-glycosylation of GDF15 abolishes its inhibitory effect on EGFR in AR inhibitor-resistant prostate cancer cells.

Wang R, Wen P, Yang G, Feng Y, Mi Y, Wang X, Zhu S, Chen YQ
Cell death & disease 2022 Jul 19;13(7):626

RNA-binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53.

Lin X, Zhou L, Zhong J, Zhong L, Zhang R, Kang T, Wu Y
The Journal of biological chemistry 2022 Feb;298(2):101524

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Jie W, Rui-Fen Z, Zhong-Xiang H, Yan W, Wei-Na L, Yong-Ping M, Jing S, Jing-Yi C, Wan-Hong L, Xiao-Hua H, Zhi L, Yan S
Virulence 2022 Dec;13(1):342-354

Global crotonylome reveals hypoxia-mediated lamin A crotonylation regulated by HDAC6 in liver cancer.

Zhang D, Tang J, Xu Y, Huang X, Wang Y, Jin X, Wu G, Liu P
Cell death & disease 2022 Aug 17;13(8):717

Discovery and Verification of Key Liver Cancer Genes and Alternative Splicing Events Based on Second-Generation Sequencing Data Analysis.

Cui M, Bai M, Zheng L, Bao Y, Sun L, Yu C, Sun Y, Song Z, Wang G, Yu Z, Li Y, Huang Y
Biological & pharmaceutical bulletin 2021;44(10):1433-1444

Therapeutic Potential of CUDC-907 (Fimepinostat) for Hepatocarcinoma Treatment Revealed by Tumor Spheroids-Based Drug Screening.

Liao W, Yang W, Xu J, Yan Z, Pan M, Xu X, Zhou S, Zhu Y, Lan J, Zeng M, Han X, Li S, Li Y, Liang K, Gao Y, Peng Q
Frontiers in pharmacology 2021;12:658197

Rational design and synthesis of 6-aryl-6H-benzo[c]chromenes as non-steroidal progesterone receptor antagonists for use against cancers.

Qin J, Qu S, Zhu K, Cheng Y, Pan G, Jing W, Liu X, Sun X, Liu L
Bioorganic & medicinal chemistry 2021 Feb 15;32:116003

Cyanidin-3-O-glucoside inhibits epithelial-to-mesenchymal transition, and migration and invasion of breast cancer cells by upregulating KLF4.

Chen D, Yuan M, Ye Q, Wang X, Xu J, Shi G, Hu Z
Food & nutrition research 2020;64

S100A6 promotes proliferation and migration of HepG2 cells via increased ubiquitin-dependent degradation of p53.

Song D, Xu B, Shi D, Li S, Cai Y
Open medicine (Warsaw, Poland) 2020;15(1):317-326

GBP1 promotes erlotinib resistance via PGK1‑activated EMT signaling in non‑small cell lung cancer.

Cheng L, Gou L, Wei T, Zhang J
International journal of oncology 2020 Sep;57(3):858-870

Bovine Milk Exosomes Affect Proliferation and Protect Macrophages against Cisplatin-Induced Cytotoxicity.

Matic S, D'Souza DH, Wu T, Pangloli P, Dia VP
Immunological investigations 2020 Oct;49(7):711-725

Combination of N, N'-dicyclohexyl-N-arachidonic acylurea and tacrolimus prolongs cardiac allograft survival in mice.

An K, Qin Q, Yu S, Xue M, Wang Z, Lin Q, Ma Y, Yan G, Mo S, Chen Y, Zhang L, Zhong J, Qi Z, Xia J
Immunology and cell biology 2020 May;98(5):382-396

Yeast Extract Induces Apoptosis and Cell Cycle Arrest via Activating p38 Signal Pathway in Colorectal Cancer Cells.

Moon D, Kang HK, Kim J, Yoon SP
Annals of clinical and laboratory science 2020 Jan;50(1):31-44

SOX6 suppresses the development of lung adenocarcinoma by regulating expression of p53, p21(CIPI) , cyclin D1 and β-catenin.

Lv L, Zhou M, Zhang J, Liu F, Qi L, Zhang S, Bi Y, Yu Y
FEBS open bio 2020 Jan;10(1):135-146

Yeast extract inhibits the proliferation of renal cell carcinoma cells via regulation of iron metabolism.

Moon D, Kim J, Yoon SP
Molecular medicine reports 2019 Oct;20(4):3933-3941

CircRNA_014511 affects the radiosensitivity of bone marrow mesenchymal stem cells by binding to miR-29b-2-5p.

Wang Y, Zhang J, Li J, Gui R, Nie X, Huang R
Bosnian journal of basic medical sciences 2019 May 20;19(2):155-163

NFIB promotes cell survival by directly suppressing p21 transcription in TP53-mutated triple-negative breast cancer.

Liu RZ, Vo TM, Jain S, Choi WS, Garcia E, Monckton EA, Mackey JR, Godbout R
The Journal of pathology 2019 Feb;247(2):186-198

HOXC13-AS promotes breast cancer cell growth through regulating miR-497-5p/PTEN axis.

Li X, Wang Q, Rui Y, Zhang C, Wang W, Gu J, Tang J, Ding Y
Journal of cellular physiology 2019 Dec;234(12):22343-22351

GTSE1 is involved in breast cancer progression in p53 mutation-dependent manner.

Lin F, Xie YJ, Zhang XK, Huang TJ, Xu HF, Mei Y, Liang H, Hu H, Lin ST, Luo FF, Lang YH, Peng LX, Qian CN, Huang BJ
Journal of experimental & clinical cancer research : CR 2019 Apr 8;38(1):152

Ginsenoside Rg1 ameliorates testicular senescence changes in D‑gal‑induced aging mice via anti‑inflammatory and antioxidative mechanisms.

Wang ZL, Chen LB, Qiu Z, Chen XB, Liu Y, Li J, Wang L, Wang YP
Molecular medicine reports 2018 May;17(5):6269-6276

MiRNA-20a-5p promotes the growth of triple-negative breast cancer cells through targeting RUNX3.

Bai X, Han G, Liu Y, Jiang H, He Q
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Jul;103:1482-1489

Knockdown of USP39 induces cell cycle arrest and apoptosis in melanoma.

Zhao Y, Zhang B, Lei Y, Sun J, Zhang Y, Yang S, Zhang X
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Oct;37(10):13167-13176

GAS5 Inhibits Gastric Cancer Cell Proliferation Partly by Modulating CDK6.

Guo X, Deng K, Wang H, Xia J, Shan T, Liang Z, Yao L, Jin S
Oncology research and treatment 2015;38(7-8):362-6

Transgenic mice overexpressing glia maturation factor-β, an oxidative stress inducible gene, show premature aging due to Zmpste24 down-regulation.

Imai R, Asai K, Hanai J, Takenaka M
Aging 2015 Jul;7(7):486-99

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 60214-1-Ig(P21 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using CDKN1A antibody 60214-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
Sample type: cell line