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LearnAb119615
antibody from Aladdin Scientific
Targeting: CDKN1A
CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- Ab119615 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant p21 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human p21 Antibody, Recombinant, could be used for WB and so on.Application:WB: 1/500-1/1000Protein FunctionMay be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 148-164- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1,5 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant p21 Antibody (Ab119615) - Western Blot All lanes: Recombinant p21 Antibody (Ab119615) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 20 kDa Exposure time: 6.6 s
