MA5-12764

antibody from Invitrogen Antibodies

Targeting: CDKN1A CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1

Immunocytochemistry

Immunoprecipitation

Antibody data

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Validation data

Product number: MA5-12764 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-p21 Monoclonal Antibody (DCS-60.2)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MA5-12764 targets p21WAF1 in IF, IHC (P), and IP applications and shows reactivity with Human samples. The MA5-12764 immunogen is full length human recombinant p21 protein.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: DCS-60.2
Vial size: 500 µL
Concentration: 0.2 mg/mL
Storage: 4° C

CDKN1A protein structure - MA5-12764 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of p21/Cip1 (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a p21/Cip1 monoclonal antibody (Product # MA5-12764) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of p21/Cip1 (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a p21/Cip1 monoclonal antibody (Product # MA5-12764) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of p21WAF1 using p21WAF1 Monoclonal Antibody (MA5-12764) on Native Human LS174T Cells.